Fluorescent polarization immunoassay utilizing substituted triazinylaminofluoresceins
Patent 4420568 Issued on December 13, 1983. Estimated Expiration Date: 
November 30, 2001. Estimated Expiration Date is calculated based on simple USPTO term provisions. It does not account for terminal disclaimers, term adjustments, failure to pay maintenance fees, or other factors which might affect the term of a patent.
November 30, 2001. Estimated Expiration Date is calculated based on simple USPTO term provisions. It does not account for terminal disclaimers, term adjustments, failure to pay maintenance fees, or other factors which might affect the term of a patent.Patent References 2937186 3223705 T3 Uptake polarization fluoroimmunoassay Patent #: 4347059 InventorsAssigneeApplicationNo. 06/325872 filed on 11/30/1981US Classes:436/536, INVOLVING IMMUNE COMPLEX FORMED IN LIQUID PHASE436/546, Fluorescent label436/800, FLUORESCENT DYES (E.G., RHODAMINE, ETC.)436/817, Steroids or hormones549/223Xanthene ring system having a benzene ring bonded directly thereto para to the xanthene ring oxygen, wherein the carbon of a -COO- group is bonded directly to the benzene ring ortho to its position of attachment to the xanthene ring system, and spirolactone derivatives thereof (e.g., fluoresceins, chlorofluorans, etc.)ExaminersPrimary: Marantz, SidneyAttorney, Agent or FirmInternational ClassesG01N 33/533 (20060101)G01N 33/533 (20060101) C07H 15/00 (20060101) C07H 15/00 (20060101) C07H 15/236 (20060101) C07H 15/236 (20060101) C07D 473/00 (20060101) C07D 473/00 (20060101) C07D 473/08 (20060101) C07D 473/08 (20060101) AbstractAn immunochemical method and reagent compounds for determining ligands in a sample. The reagent compounds are derivatives of a triazinylaminofluorescein and are represented by the structural formula ##STR1## wherein Y is halo or lower alkyl; andR is a ligand-analog wherein said ligand-analog has at least one common epitope with said ligand so as to be specifically recognizable by a common antibody.The reagent compound and an antibody specific to the ligand are added to the sample in a fluorescence polarization immunoassay. |
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