Processes for inserting DNA into eucaryotic cells and for producing proteinaceous materials
Patent 4399216 Issued on August 16, 1983. Estimated Expiration Date: 
August 16, 2000. Estimated Expiration Date is calculated based on simple USPTO term provisions. It does not account for terminal disclaimers, term adjustments, failure to pay maintenance fees, or other factors which might affect the term of a patent.
August 16, 2000. Estimated Expiration Date is calculated based on simple USPTO term provisions. It does not account for terminal disclaimers, term adjustments, failure to pay maintenance fees, or other factors which might affect the term of a patent.Patent References 3800035 Process for obtaining insulin-producing animal cells Patent #: 4195125 InventorsApplicationNo. 06/124513 filed on 02/25/1980US Classes:435/6, Involving nucleic acid435/29, Involving viable micro-organism435/320.1, VECTOR, PER SE (E.G., PLASMID, HYBRID PLASMID, COSMID, VIRAL VECTOR, BACTERIOPHAGE VECTOR, ETC.) BACTERIOPHAGE VECTOR, ETC.)435/34, Determining presence or kind of micro-organism; use of selective media435/356, L cell or derivative (e.g., Ltk(-), etc.)435/357, Fibroblast, fibroblast-like cell or derivative (e.g., NIH 3T3, etc.)435/358, Chinese hamster ovary (i.e., CHO)435/360, Expressing recombinant hormone or growth factor435/465, Involving co-transfection435/69.1, Recombinant DNA technique included in method of making a protein or polypeptide435/69.3, Antigens435/69.4, Hormones and fragments thereof435/69.51, Interferons435/69.6, Blood proteins435/811, INTERFERON435/948, Using viruses or cell lines536/23.1DNA or RNA fragments or modified forms thereof (e.g., genes, etc.)ExaminersPrimary: Kepplinger, Esther M.Attorney, Agent or FirmInternational ClassesC12P 21/02 (20060101)C12N 15/85 (20060101) C12N 15/87 (20060101) AbstractThe present invention relates to processes for inserting DNA into eucaryotic cells, particularly DNA which includes a gene or genes coding for desired proteinaceous materials for which no selective criteria exist. The insertion of such DNA molecules is accomplished by cotransforming eucaryotic cells with such DNA together with a second DNA which corresponds to a gene coding for a selectable marker.The invention further relates to processes for inserting into eucaryotic cells a multiplicity of DNA molecules including genes coding for desired proteinaceous materials by cotransformation with the desired genes and with amplifiable genes for a dominant selectable marker in the presence of successively higher amounts of an inhibitor. Alternatively, the insertion of multiple copies of desired genes is accomplished by transformation using DNA molecules formed by ligating a DNA molecule including the desired gene to a DNA molecule which includes an amplifiable gene coding for a dominant selectable phenotype such as a gene associated with resistance to a drug in the presence of successively higher amounts of an agent such as a drug against which the gene confers resistance so that only those eucaryotic cells into which multiple copies of the amplifiable gene have been inserted survive.Other References
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