Agglutination tests for detecting influenza viruses, and reagents for carrying out these tests

Patent 4355102 Issued on October 19, 1982. Estimated Expiration Date:

March 5, 2000. Estimated Expiration Date is calculated based on simple USPTO term provisions. It does not account for terminal disclaimers, term adjustments, failure to pay maintenance fees, or other factors which might affect the term of a patent.

Abstract Claims Description Full Text

Patent References

3826613

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Patent #: 3963441
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Inventor

Quash, Gerard

Application

No. 06/128232 filed on 03/05/1980

US Classes:

435/5, Involving virus or bacteriophage435/174, CARRIER-BOUND OR IMMOBILIZED ENZYME OR MICROBIAL CELL; CARRIER-BOUND OR IMMOBILIZED CELL; PREPARATION THEREOF435/18, Involving hydrolase435/810PACKAGED DEVICE OR KIT

Examiners

Primary: Wiseman, Thomas G.

Attorney, Agent or Firm

Browdy and neimark

International Classes

C12Q 1/34 (20060101)
C12Q 1/70 (20060101)
G01N 33/544 (20060101)
G01N 33/569 (20060101)
G01N 33/543 (20060101)
G01N 33/548 (20060101)

Foreign Application Priority Data

1979-03-06 FR

Abstract

The present invention relates to a new test for detecting influenza viruses. This test comprises: 1- the addition of influenzia virus or of allantoic liquid containing said virus to neuraminidase substrate containing at least one sialic acid group. The substrate is fixed by covalent coupling to an appropriate insoluble solid support, it being understood that said substrate may be formed by all types of mucoproteins, oligosaccharides, mucopolysaccharides or mucolipids comprising at least one sialic acid group capable of being used as a neuraminidase substrate; 2- incubation of the influenza virus with its substrate for an appropriate length of time; 3- then reading the reaction.

Other References

Quash, "Biological Reactants Consisting of Solid Supports to which are Coed Organic Compounds Containing Carbohydrate Residues", Chem. Absts., vol. 90 (1979), Absts. No. 148119z
Holmquist, "Preparation of Sepharose-Bound ଱-Ketosides of N-Acetylneuraminic Acid and their Interaction with Vibrio cholerae Neuraminidase, Chem. Absts., vol. 82, No. 11, (1975), p. 158, Abs. No. 69667h
Thraenhart, et al., "Standardization of a Rapid Modified Micro-Neuraminidase-Inhibition Test (Essen-NIT) for Influenza Virus-Neuraminidase Antibody Assay and Comparison with W.H.O. Method", J. Biol. Stand., vol. 4 (1976). pp. 225-241
Santer, et al., "A Rapid Assay for Neuraminidase", Brochim. Biophys. Acta., vol. 523, (1978), pp. 435-442
Cuatrecasas et al., "Purification of Neuraminidases from Vibrio Cholerae, Clostridium Perfringens and Influenzia Virus by Affinity Chromatography", Biochem. Biophys. Res. Comm., vol. 44, (1971), No. 1, pp. 178-184
Warren, "The Thiobarbituric Acid Assay of Sialic Acids", J. Biol. Chem., vol. 234, No. 8, (1959), pp. 1971-1975