Method in assaying methods involving biospecific affinity reactions and reagent for use in said method
Patent 4231999 Issued on November 4, 1980. Estimated Expiration Date: 
March 2, 1998. Estimated Expiration Date is calculated based on simple USPTO term provisions. It does not account for terminal disclaimers, term adjustments, failure to pay maintenance fees, or other factors which might affect the term of a patent.
March 2, 1998. Estimated Expiration Date is calculated based on simple USPTO term provisions. It does not account for terminal disclaimers, term adjustments, failure to pay maintenance fees, or other factors which might affect the term of a patent.Patent References 3896217 Method for detection of biological particles Radioimmunoassay system Automated direct serum radioimmunoassay Patent #: 4108976 InventorsAssigneeApplicationNo. 05/882678 filed on 03/02/1978US Classes:435/7.8, Involving nonmembrane bound receptor binding or protein binding other than antigen-antibody binding435/7.9, Assay in which an enzyme present is a label435/7.92, Heterogeneous or solid phase assay system (e.g., ELISA, etc.)436/518, INVOLVING AN INSOLUBLE CARRIER FOR IMMOBILIZING IMMUNOCHEMICALS436/800, FLUORESCENT DYES (E.G., RHODAMINE, ETC.)436/804RADIOISOTOPE (E.G., RADIOIMMUNOASSAY, ETC.)ExaminersPrimary: Padgett, Benjamin R.Assistant: Nucker, Christine M. Attorney, Agent or FirmInternational ClassG01N 33/543 (20060101)AbstractA method of carrying out assaying methods involving biospecific affinity reactions in which there is used a component (I), which is labelled with at least one analytically indicatable group and is soluble in the liquid in whose presence the biospecific affinity reaction is carried out, for reaction with a counterpart (II) which exhibits biospecific affinity to (I) and optionally also one or more additional counterparts exhibiting biospecific affinity to (I) and/or (II), and forming an insoluble conjugate in which the labelled component (I) is incorporated and which conjugate is separated from the liquid phase, whereafter the analytically indicatable group is assayed in the insoluble conjugate, said method being characterized in that the analytically indicatable group is bound to the component (I) with a splittable bond of covalent nature and/or one of said counterparts is bound to a carrier, which is insoluble in said liquid, with a splittable bond of covalent nature, said splittable bonds being splittable under conditions which have practically disturbing influence on the assay method used; and in that the insoluble conjugate is split at said splittable bond or bonds, in the presence of a liquid, to form fragments which are soluble in said liquid and which contain the analytically indicatable group, which group is then assayed in the liquid phase. |
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