U.S. patents available from 1976 to present.
U.S. patent applications available from 2005 to present.

Methods for the detection and determination of ligands

Patent 4228237 Issued on October 14, 1980. Estimated Expiration Date: Icon_subject September 21, 1998. Estimated Expiration Date is calculated based on simple USPTO term provisions. It does not account for terminal disclaimers, term adjustments, failure to pay maintenance fees, or other factors which might affect the term of a patent.

Patent References

Enzyme conjugates
Patent #: 4002532
Issued on: 01/11/1977
Inventor: Weltman ,   et al.

Enzymatic immunological method for the determination of antigens and antibodies
Patent #: 4016043
Issued on: 04/05/1977
Inventor: Schuurs, et al.

Specific binding assay with an enzyme modulator as a labeling substance Patent #: 4134792
Issued on: 01/16/1979
Inventor: Boguslaski ,   et al.

Inventors

Application

No. 05/944254 filed on 09/21/1978

US Classes:

435/5, Involving virus or bacteriophage435/188, Stablizing an enzyme by forming a mixture, an adduct or a composition, or formation of an adduct or enzyme conjugate435/7.5, Involving avidin-biotin binding435/7.93, Competitive assay435/7.94, Sandwich assay436/513, INVOLVING IGA, IGD, IGE, OR IGM436/518, INVOLVING AN INSOLUBLE CARRIER FOR IMMOBILIZING IMMUNOCHEMICALS436/547, INVOLVING PRODUCTION OR TREATMENT OF ANTIBODY436/810, Tube, bottle, or dipstick436/815TEST FOR NAMED COMPOUND OR CLASS OF COMPOUNDS

Examiners

Primary: Wiseman, Thomas G.

Attorney, Agent or Firm

International Classes

G01N 33/543 (20060101)
G01N 33/531 (20060101)
G01N 33/532 (20060101)

Abstract

A method for determining the presence of a ligand in a liquid medium which utilizes enzyme labeled avidin and biotin labeled reagent in a specific binding process wherein the ligand to be detected is contacted with an insoluble phase containing specific binding substance for the ligand. Various protocols may be used to assay the resulting enzyme activity which is related to the amount of ligand in the liquid medium.

Other References

  • May, et al., "N&3xb5;B29 -(+)-Biothylinsulin and its Complexes with Audin", J. Biol Chem., vol. 253, No. 3, (1978) pp. 686-690
  • Manning, et al., "A Method for Gene Enrichment Based on the Auidin-Biochem Interaction Application to the Drosophilia Ribosomul Genes," Biochem., vol. 16, No. 7 (1977), pp. 1364-1370
  • Heggeness, et al., "Use of the Avidin-Biotin Complex for the Localization of Actin and Myosin with Fluorescene Microscopy," J. Cell Biol. vol. 73 (1977), pp. 783-788
  • Hofmann, et al., "Avidin-Biotin Affinity Columns, General Methods for Attaching Biotin to Peptides and Proteins," J. Am. Chem. Soc. vol. 100, No. 11 (1978), pp. 3585-3590
  • Bayer, et al., "Preparation of Ferritin-Avidin Conjugates by Reductive Alkylation for Use in Electron Microscope Cytochemistry," J. Histochem and Cytochem, vol. 24, No. 8, (1976), pp. 933-939
  • Wisdom, "Enzyme-Immunoassay," Clin. Chem., vol. 22 No. 8, (1976), pp. 1243-1255
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