Patent ReferencesEnzyme conjugates Enzymatic immunological method for the determination of antigens and antibodies Specific binding assay with an enzyme modulator as a labeling substance Patent #: 4134792 InventorsApplicationNo. 05/944254 filed on 09/21/1978US Classes:435/5, Involving virus or bacteriophage435/188, Stablizing an enzyme by forming a mixture, an adduct or a composition, or formation of an adduct or enzyme conjugate435/7.5, Involving avidin-biotin binding435/7.93, Competitive assay435/7.94, Sandwich assay436/513, INVOLVING IGA, IGD, IGE, OR IGM436/518, INVOLVING AN INSOLUBLE CARRIER FOR IMMOBILIZING IMMUNOCHEMICALS436/547, INVOLVING PRODUCTION OR TREATMENT OF ANTIBODY436/810, Tube, bottle, or dipstick436/815TEST FOR NAMED COMPOUND OR CLASS OF COMPOUNDSExaminersPrimary: Wiseman, Thomas G.Attorney, Agent or FirmInternational ClassesG01N 33/543 (20060101)G01N 33/531 (20060101) G01N 33/532 (20060101) AbstractA method for determining the presence of a ligand in a liquid medium which utilizes enzyme labeled avidin and biotin labeled reagent in a specific binding process wherein the ligand to be detected is contacted with an insoluble phase containing specific binding substance for the ligand. Various protocols may be used to assay the resulting enzyme activity which is related to the amount of ligand in the liquid medium.Other References
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