Process for extracting and processing glycoproteins, mucopolysaccharides and accompanying substances Patent #: 4108849
ApplicationNo. 05/903006 filed on 05/04/1978
US Classes:436/543, INVOLVING PRODUCING OR TREATING ANTIGEN OR HAPTEN424/531, Serum424/583, Placenta or umbilical cord435/212, Acting on peptide bond (e.g., thromboplastin, leucine amino-peptidase, etc., (3.4))435/217, Plasmin (i.e., fibrinolysin)435/219, Proteinase435/226, Derived from animal tissue (e.g., rennin, etc.)436/547, INVOLVING PRODUCTION OR TREATMENT OF ANTIBODY436/814, Pregnancy530/386, Globulins530/392, Alpha globulins, e.g., ceruloplastin, haptoglobins, etc.530/395, Glycoprotein, e.g., mucins proteoglycans, etc.530/412, Separation or purification530/413, Immunological separation or affinity chromatography530/416, Ion exchange530/830, Plasma; serum530/851Placenta; amniotic fluid
ExaminersPrimary: Shapiro, Lionel M.
Attorney, Agent or Firm
International ClassesA61K 35/16 (20060101)
A61K 35/48 (20060101)
A61K 35/50 (20060101)
C07K 14/47 (20060101)
C07K 14/435 (20060101)
G01N 33/531 (20060101)
A61K 38/00 (20060101)
A61K 39/00 (20060101)
Foreign Application Priority Data1977-05-07 DE
AbstractWhat is disclosed is a glycoprotein having proteolytic, fibrinolytic, and thrombolytic properties and which can be used to prepare anti-sera useful as diagnostic agents, said glycoprotein being found in human blood serum and extracts of human placentas and being capable of isolation therefrom and which has the following properties:a protein proportion essentially consisting of 89b1;4% of -amino-acids,a carbohydrate proportion of 11.1b1;2.2%, among it 5.3b1;1.1% of hexoses, 2.8b1;0.5 N of N-acetylated hexosamine, 2.9b1;0.6% of N-acetylated neuraminic acid;a sedimentation coefficient S20x of 3.2b1;0.3 S;a molecular weight of 32 000b1;6 000;an iso-electric point at pH 4.3b1;0.3;an extinction coefficient E1 cm1% (280 nm) of 13.8b1;1.0;an electrophoretic mobility in the range between that of albumin and 댑 -globulins;a specific immunologic reaction with an antibody specifically directed against the protein, anda proteolytic activity, as well as a process for isolating it.