Compounds for enzyme amplification assay
Patent 4067774 Issued on January 10, 1978. Estimated Expiration Date: 
January 10, 1995. Estimated Expiration Date is calculated based on simple USPTO term provisions. It does not account for terminal disclaimers, term adjustments, failure to pay maintenance fees, or other factors which might affect the term of a patent.
January 10, 1995. Estimated Expiration Date is calculated based on simple USPTO term provisions. It does not account for terminal disclaimers, term adjustments, failure to pay maintenance fees, or other factors which might affect the term of a patent.Patent References3878187 InventorAssigneeApplicationNo. 05/722964 filed on 09/13/1976US Classes:435/188, Stablizing an enzyme by forming a mixture, an adduct or a composition, or formation of an adduct or enzyme conjugate435/189, Oxidoreductase (1. ) (e.g., luciferase)435/190, Acting on CHOH group as donor (e.g., glucose oxidase, lactate dehydrogenase (1.1))435/195, Hydrolase (3. )435/7.9, Assay in which an enzyme present is a label435/964, INCLUDING ENZYME-LIGAND CONJUGATE PRODUCTION (E.G., REDUCING RATE OF NONPRODUCTIVE LINKAGE, ETC.)930/260, Containing Cys-Cys disulfide bridge between nonadjacent cysteine residues (See note 7)930/40Angiotensin; related peptidesExaminersPrimary: Tanenholtz, Alvin E.Attorney, Agent or FirmAbstractNovel biological assay method for determining the presence of a specific organic material by employing a modified enzyme for amplification. By employing receptors specific for one or a group of materials (hereinafter referred to as "ligands") and binding an enzyme to the ligand or ligand counterfeit to provide an "enzyme-bound-ligand", an extremely sensitive method is provided for assaying for ligands. The receptor when bound to the enzyme-bound-ligand substantially inhibits enzymatic activity, providing for different catalytic efficiencies of enzyme-bound-ligand and enzyme-bound-ligand combined with receptor.The receptor, ligand and enzyme-bound-ligand are combined in an arbitrary order and the effect of the presence of ligand on enzymatic activity determined. Various protocols may be used for assaying for enzymatic activity and relating the result to the amount of ligand present. |
| ||||||||||||||
