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Class 530/412 - Separation or purification


Subclass of Class 530 - Chemistry: natural resins or derivatives; peptides or proteins; lignins or reaction products thereof
Definition: Processes in which a protein is separated from another material.
No. of patents: 1438
Last issue date: 04/24/2012


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NumberTitleIssue Date
8163890Production of recombinant proteins by autoproteolytic cleavage of a fusion protein
Disclosed is a method for the production of a heterologous polypeptide of interest with a homogenous N-terminus, using a fusion polypeptide comprising the polypeptide of interest and N-terminally thereto a polypeptide exhibiting autoproteolytic function, said method...
04/24/2012
8148502R-ALPHA1 antitrypsin anion exchange chromatography
The present invention relates to a method for obtaining highly purified recombinant alpha1-antitrypsin (rAAT) using anion exchange chromatography, wherein a composition comprising rAAT and at least one impurity originating from cultivation of cells used for generati...
04/03/2012
8138317Purification of pegylated polypeptides
The invention is a method for the purification of mono-PEGylated erythropoietin using two cation exchange chromatography steps wherein the same type of cation exchange material is used in both cation exchange chromatography steps and a method for producing a mono-PE...
03/20/2012
8129507Atopic dermatitis inducer
An atopic dermatitis inducer binding to a human own IgE antibody and activating mast cells and basophiles, which includes a purified human secretion fraction, or an antigenic molecule or an antigenic determinant in the purified fraction, and obtained through the fol...
03/06/2012
8097586Modified exedins and uses thereof
Novel exendins with modifications at one or more of following positions: 2, 14, 27 or 28 and polyethylene glycol derivatives thereof are provided. These compounds are useful in treating type 2 diabetes as GLP-1 receptor agonists. ...
01/17/2012
8084587Method for the production of proteins
The present invention relates to a process for the purification of a protease. ...
12/27/2011
8067552Method for preparing a target protein using the sHSPs
The present invention relates to a method for separating and purifying a target protein, a method for preparing a target protein, and a method for bioconversion by a whole cell enzyme or a partially purified enzyme. According to the present invention, when the sHSPs...
11/29/2011
8067553PEG-urate oxidase conjugates and use thereof
A naturally occurring or recombinant urate oxidase (uricase) covalently coupled to poly(ethylene glycol) or poly(ethylene oxide) (both referred to as PEG), wherein an average of 2 to 10 strands of PEG are conjugated to each uricase subunit and the PEG has an average...
11/29/2011
8034907Polynucleotides encoding soluble, stable forms of human double minute 2 polypeptides
The present invention discloses modified Hdm2 proteins that are soluble. In addition, the present invention discloses nucleic acids that encode the modified Hdm2 proteins of the present invention. The invention also provides crystals of modified Hdm2 proteins that a...
10/11/2011
8013129Protein separation device
The invention provides a protein separation device comprising a chaperone protein immobilized on a substrate. In one embodiment, the chaperone protein is an Hsp60 chaperone, preferably a group one chaperone, preferably GroEL. The invention also provides a method for...
09/06/2011
7999084Devices and methods for reducing matrix effects
Devices and methods are provided for reducing matrix effects in protein precipitated bioanalytical samples comprising: a support, and a sorbent associated with the support capable of binding matrix interfering agents present in the bioanalytical sample, wherein the ...
08/16/2011
7985846Hemostatically active vWF-containing preparation and process for the preparation thereof
A process for preparing a hemostatically active preparation containing von Willebrand factor (vWF) from a fraction of human plasma by chromatographic purification of a vWF-containing plasma fraction on an anion-exchange material which has the anion-exchanging groups...
07/26/2011
7960519Extraction of cellular components with fatty acid derivatives
A method of treating biologic material with a composition containing at least one fatty acid derivative and/or at least one fatty alcohol, a buffer, and a salt to result in protein partitioning into an aqueous phase and an organic phase. The protein may be a membran...
06/14/2011
7956166Fractionation of protein containing mixtures
Thus, a primary aspect of the present invention relates to a method for the fractionation of a protein-containing mixture wherein the protein-containing mixture is selected from the group consisting of milk, milk derived products, milk derived raw materials, vegetab...
06/07/2011
7956165Compositions and methods for purifying and crystallizing molecules of interest
A composition-of-matter is provided. The composition comprising at least one antibody binding moiety capable of binding an antibody-labeled target molecule, cell or virus of interest, said at least one antibody binding moiety being attached to at least one coordinat...
06/07/2011
7956167Purification of collagenases from liquid culture
The present invention provides a method for purifying Clostridium histolyticum collagenase type I and type II proteins from a complex mixture by subsequently performing a precipitation with ammonium sulfate, hydrophobic interaction chromatography, cation exch...
06/07/2011
7943746Process for the purification of IL-18 binding protein
The invention relates to a process for the purification of IL-18 binding protein (IL-18BP) from a fluid using aqueous two-phase partitioning. ...
05/17/2011
7935798Method for the extraction of intracellular proteins from a fermentation broth
A method for extracting an intracellular peptide, protein or other polypeptide from a whole fermentation broth using a water miscible alcohol, or a water miscible or partially water miscible glycol ether. ...
05/03/2011
7932365Preparation and composition of inter-alpha inhibitor proteins from human plasma for therapeutic use
The invention relates to Inter-alpha inhibitor proteins (IαIp). The invention further relates to processes for purification of IαIp compositions and their use for treatment of human diseases such as sepsis and septic shock, rheumatoid arthritis, cancer and infecti...
04/26/2011
7919597Method of producing cell extract for cell-free protein synthesis
A cell extract for cell-free protein synthesis is produced by removing substances, which bind to an affinity support to be used in purification or interaction analysis, from a cell extract having protein synthetic activity. Then, a target protein is synthesized by u...
04/05/2011
7897734Method for the production of proteins
The present invention relates to a process for the purification of a protease. ...
03/01/2011
7868144Method for unspecific enrichment of bacterial cells
The present invention relates to a method for unspecific enrichment of bacterial cells by means of cationic or anionic polymers and magnetic carriers. ...
01/11/2011
7868145Magnetic particles containing a copolymer core, magnetic layer and silicon layer
A magnetic particle and fabrication method thereof. The magnetic particle comprises a polymer core, a magnetic material layer covering the polymer core, and a silicon containing layer covering the magnetic material layer. In addition, the magnetic particle may furth...
01/11/2011
7868146Method and device for producing a thread from silk proteins
The present invention relates to a thread preparation process from silk proteins including an apparatus which is appropriate for performing the method. Furthermore, the invention is directed to the threads obtained therewith as well as the use thereof. The invention...
01/11/2011
7855280Separation methods
Separation methods, for example, to isolate a recombinant protein, are disclosed. In some implementations, a method includes forming a solid containing a first cation and a first anion in a medium containing a protein, and separating the solid from the protein. ...
12/21/2010
7838640Method of producing cell extract for cell-free protein synthesis
A cell extract for cell-free protein synthesis is produced by removing substances, which bind to an affinity support to be used in purification or interaction analysis, from a cell extract having protein synthetic activity. Then, a target protein is synthesized by u...
11/23/2010
7834161Process for proteolytic cleavage and purification of recombinant proteins produced in plants
The present invention relates to improved methods for protein purification of high-value heterologous proteins by providing fusion proteins suitable for affinity purification and improved and economical methods of proteolytic cleavage of fusion proteins. The methods...
11/16/2010
7829680System and method for isolation of gluten as a co-product of ethanol production
The present invention is a method for protein isolation as a co-product of alcohol production. The method includes receiving grain product at a holding receptacle, the grain product being at least one of whole stillage, dried distillers grain, and wet distillers gra...
11/09/2010
7825227Method for purification of a protein complex and identification of its components
The present invention provides a method for purifying a protein complex and the components comprising the complex from a cell, a cell or tissue lysate or a whole organism by employing a combined set of affinity tags of high affinity, specificity and ease of elution....
11/02/2010
7812138Fractionation of protein containing mixtures
Thus, a primary aspect of the present invention relates to a method for the fractionation of a protein-containing mixture wherein the protein-containing mixture is selected from the group consisting of milk, milk derived products, milk derived raw materials, vegetab...
10/12/2010
7795405Procedure for the fractionation of proteins by using sequential ion exchange and hydrophobic interaction chromatography as prefractionation steps before analysis by two dimensional electrophoresis
After the sequencing of the human genome, great interest has developed in trying to discern the complementary proteome of humans and other species. The present disclosure provides devices, systems, and methods for proteomic fractionation that may increase the number...
09/14/2010
7763717Continuous protein and lipid recovery from food animal processing byproducts
A process and system for recovering protein and lipid from food animal byproducts, and the products thereof, involves homogenizing animal byproducts with water to form a homogenate, solubilizing the homogenate by adjusting the pH of the homogenate to form a first pH...
07/27/2010
7754861Method for purifying proteins
The present invention provides methods and kits for purifying a target protein group. The method comprises the steps of contacting a sample comprising at least 95% of the target protein group and at most 5% of contaminating proteins with a library of binding moietie...
07/13/2010
7754860Method for purifying FSH
The invention provides a method for purifying recombinant FSH. ...
07/13/2010
7741455Method for purifying FSH
The invention relates to a method for purifying recombinant human FSH or an FSH variant starting from crude FSH, comprising the following steps: 1) dye-affinity chromatography; 2) hydrophobic interaction chromatography; and 3) reverse phase chromatography. ...
06/22/2010
7728116Method of preparing an osteogenic protein fraction
In a method of isolating osteogenic protein from bone, in which an osteogenic protein-containing fraction is extracted from bone and enriched by a sequence of enrichment steps selected from ultrafiltration and chromatography, the invention provides the improvement o...
06/01/2010
7723492Method for the detection of sepsis using procalcitonin-3-116
Uses of recombinant procalcitonin 3-116 in the diagnosis and therapy of septic diseases and the measurement of prohormones other than procalcitonin, and of dipeptidyl peptidase IV, as biomarkers in the diagnosis of sepsis. ...
05/25/2010
7723491Methods of isolating specific compounds from supramammary lymph node tissue
Methods of forming proteinaceous products such as cell culture supplement capable of supporting the growth and culturing of cells, tissue, and organs, are generally disclosed. One method generally provides for the collection of the internal tissue of an animal, such...
05/25/2010
7723490Process for correction of a disulfide misfold in Fc molecules
The present invention concerns a process by which a misfold in an Fc fusion molecule can be prevented or corrected. In one embodiment, the process comprises (a) preparing a pharmacologically active compound comprising an Fc domain; (b) treating the fusion molecule w...
05/25/2010
7700744Method for isolating proteins or protein and nucleic acid associations, or particle and protein complexes, reagent and uses
The magnetic colloidal particles comprise a core and an envelope in which the core is magnetic and is coated with at least one polymer comprising functional groups X chosen from amine, hydroxyl, thiol, aldehyde, ester, anhydride, acid chloride, carbonate, carbamate,...
04/20/2010
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