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| Number | Title | Issue Date |
| 7183110 | Antibody immunoreactive with a 5-enolpyruvylshikimate-3-phosphate synthase Genes encoding Class II EPSPS enzymes are disclosed. The genes are useful in producing transformed bacteria and plants which are tolerant to glyphosate herbicide. Class II EPSPS genes share little homology with known, Class I EPSPS genes, and do not hybridize to pro... | 02/27/2007 |
| 7145044 | Method of producing reduced coenzyme Qusing solvent with high oxidation-protective effect The present invention relates to a method of conveniently and efficiently producing reduced coenzyme Q10 having excellent qualities which is useful in foods, functional nutritive foods, specific health foods, nutritional supplements, nutrients, drinks, fe... | 12/05/2006 |
| 7060478 | Process for preparing lysozyme A process for preparing lysozyme is provided which is characterized by mixing the egg white or its diluted solution with diatomaceous earth, kaolin, zeolite, or the mixtures thereof, and followed by eluting the adsorbed lysozyme with a salt solution. The diatomaceou... | 06/13/2006 |
| 7019120 | Cloud-point extraction of enzymes and polypeptides from a fermentation broth using a non-ionic surfactant A method of extracting a polypeptide of interest from a fermentation broth comprising: i) adjusting the pH close to pI of the polypeptide of interest; ii) adding a non-ionic surfactant with a hydrophile-lipophile b... | 03/28/2006 |
| 6825027 | Method of production of purified hepatitis a virus particles and vaccine preparation The present invention provides methods of purification of Hepatitis A Virus from the supernatant of an infected cell culture and production of a preparation of purified HAV antigen. The present invention is also directed to an HAV vaccine composition comprising a pr... | 11/30/2004 |
| 6630338 | Removing deacetylase contaminant from cephalosporin C acylase using cationic surfactant An enzyme in a mixture containing the enzyme and a contaminant enzyme is purified by selectively aggregating and precipitating the contaminant enzyme with a surfactant. A deacetylase contaminant is separated from Cephalosporin C acylase using a cationic s... | 10/07/2003 |
| 6593118 | Method for rapidly obtaining crystals with desirable morphologies The present invention provides a crystallization process wherein a starting temperature is selected such that a desirable crystal morphology (e.g., square) is obtained. A temperature shift is then introduced, providing that the shift is not enough to indu... | 07/15/2003 |
| 6566134 | High Beta-Conglycinin products and their use The utility of soybeans having a composition of greater than 40% of the protein as beta-conglycinin and less than 10% of the protein as glycinin for making highly functional high beta-conglycinin compositions was discovered. The discovered ingredients are... | 05/20/2003 |
| 6431370 | Direct enzyme agglomeration process Detergent formulations are prepared by directly agglomerating a fermentation broth extract, containing a detergent-type enzyme and a nonionic detergent-type surfactant, with a suitable detergent base mixture, without need for prior isolation of the enzyme... | 08/13/2002 |
| 6406912 | Method for enzymatic enantiomer-separation of 3(r)- and 3(s)-hydroxy-1-methyl-4-(2,4,6-trimethoxyphenyl)-1,2,3,6-tetrahydro-pyridi ne or its carboxylic acid esters The invention relates to a method for producing optically pure compounds of 3(R)- and 3(S)-hydroxy-1-methyl-4-(2,4,6-trimethoxyphenyl)-1,2,3,6-tetrahydro-pyridi ne or its carboxylic acid esters by reacting the enantiomer mixtures stereoselectively, us... | 06/18/2002 |
| 6403350 | Method for rapidly obtaining enzyme crystals with desirable morphologies The present invention provides a crystallization process wherein a starting temperature is selected such that a desirable crystal morphology (e.g., square) is obtained. A temperature shift is then introduced, providing that the shift is not enough to indu... | 06/11/2002 |
| 6165758 | Purifying cephalosporin C acylase and regenerating a carrier immobilizing cephalosporin C acylase An enzyme in a mixture containing the enzyme and a contaminant enzyme is purified by selectively aggregating and precipitating the contaminant enzyme with a surfactant. A carrier containing an immobilized enzyme is regenerated by using a protease to remov... | 12/26/2000 |
| 6105786 | Surfactant-based extraction process A process is disclosed for preparing a detergent powder containing enzymes where the enzymes are extracted from a fermentation broth with a salt and surfactant mixture and directly agglomerated with detergent paste and dried to form the detergent powder. ... | 08/22/2000 |
| 6087148 | Method of purification of cellulose from a broth solution by crystallization A method for purification, and isolation in crystalline form, of a cellulase from a broth comprises: treating the broth with a crystallization-effective amount of a water-miscible organic solvent (e.g. a lower aliphatic alcohol or ketone); and isolating t... | 07/11/2000 |
| 6066481 | Crystallization of a protein with a sulphur salt The present invention relates to a method for crystallization of a protein obtained from a protein-containing solution which involves (a) treating the protein-containing solution with a salt containing a sulphur atom having an oxidation state less than 6,... | 05/23/2000 |
| 5919684 | Process for the reduction of catalase activity in alcohol oxidase A process is disclosed for treating compositions of alcohol oxidase to inactivate catalase therein, which comprises aging the composition comprising alcohol oxidase and catalase at a temperature and for a time period sufficient to inactivate catalase whil... | 07/06/1999 |
| 5882520 | Use of arabinogalactan in aqueous two phase extractions Aqueous two phase systems are provided which permit the extractive separation of biological materials. In a preferred embodiment, an aqueous solution of arabinogalactan defines at least one phase, and an aqueous solution of a second solute, such as a poly... | 03/16/1999 |
| 5854064 | Methods for fractionation of biologically-derived materials Supercritical and near critical fluids are used to fractionate biomass materials in two steps. In the first step, the biomass is exposed to elevated pressure supercritical or near critical fluid to bring about disruption of the biomass. In the second step... | 12/29/1998 |
| 5837513 | Method for crystallization of enzymes A method for rapidly and inexpensively crystallizing enzymes from an impure mixture is disclosed. The yield of the enzyme is greater than 35% within twelve hours. The crystallizing agent is a salt added in a concentration which is less than the concentrat... | 11/17/1998 |
| 5733764 | Method for purification of an aqueous enzyme solution Particular enzymes are precipitated from a mixture of proteins by the simultaneous addition of a soluble aluminate and acid. The pH of the mixture is at least one pH unit from the isoelectric point of the particular enzyme.... | 03/31/1998 |
| 5719048 | Separation of proteins The present invention relates to a method of separating an enzyme from an aqueous solution comprising this enzyme in mixture with other proteins, and recovery of the desired enzyme on crystalline form.... | 02/17/1998 |
| 5709796 | Process for digesting cellulose containing solid wastes A process and apparatus for reducing the volume and mass of solid waste (A) by initially subjecting the solid waste to a digestive enzymatic solution (20) agitated by fluid jets (28) under conditions which convert substrate (12) into a liquid waste which ... | 01/20/1998 |
| 5491085 | Method of recovering peroxidase from seed hulls using a freeze-thaw technique Peroxidase can be recovered from seed hulls in an improved method using a freeze-thaw technique. First, the seed hulls are comminuted, placed into water and homogenized. Next, the homogenate is frozen then thawed. The enzyme is then recovered from the aqu... | 02/13/1996 |
| 5472864 | Method of preparing solution enriched in EG III using low molecular weight alcohol, organic salt and inorganic salt A method for preparing an aqueous solution enriched in EG III from an aqueous mixture containing cellulase proteins, xylanase and EG III is disclosed. The method involves adding an amount of a low molecular weight alcohol selected from the group consistin... | 12/05/1995 |
| 5445949 | Large scale separation and purification of fermentation product A method of separating a hydrophobic fermentation product selected from the group consisting of lipase, esterase, endoxylanase and an antibiotic from a mixture comprising said product and contaminants which method comprises adding to the mixture sequentia... | 08/29/1995 |
| 5439817 | Method of preparation of purified alkaline protease A highly purified alkaline protease preparation is produced by separating a mixture containing amorphous form and crystalline form of alkaline protease from ultrafiltrate containing impurities, through a method which includes the incubation of an aqueous ... | 08/08/1995 |
| 5434072 | Method for preparing an aqueous solution enriched in both EG-III & xylanase using a low molecular weight alcohol and an organic salt A method for preparing an aqueous solution enriched in xylanase only, EG III only and both EG III and xylanase from an aqueous mixture containing cellulase proteins, xylanase and EG III is disclosed. The methods involve adding an amount of a low molecular... | 07/18/1995 |
| 5432072 | Purification method for materials having nick-translation ability The present invention pertains to a method of purification of DNA polymerase I, and the polymerase and Nick-translation activities thereof. In one embodiment, the method of purification is directed to circumstances where there are amplified amounts of the... | 07/11/1995 |
| 5405767 | Purified enzyme concentrate and method of preparation A novel compound for producing a purified enzyme precipitate and a method of preparing the purified enzyme precipitate, wherein the compound is an organic compound selected from the group consisting of carboxylic acids having at least 2 carboxyl groups, s... | 04/11/1995 |
| 5360729 | Method for purification of recombinant copper/zinc (CU-ZN) superoxide dismutase from bacteria or eucaryotic cells The subject invention provides a method for recovering a solution containing purified, enzymatically active Cu-Zn superoxide dismutase or a polypeptide analog thereof having substantially the same amino acid sequence as, and the bioloical activity of, nat... | 11/01/1994 |
| 5352598 | Purified luciferase from luciola lateralis A purified luciferase from Luciola lateralis is disclosed. The enzyme is characterized by having properties including: an optimum pH range of 7.5 to 9.5, an optimum temperature range of 0° C. to 50° C., and that the enzyme does not act on ADP, CTP, UTP,... | 10/04/1994 |
| 5326477 | Process for digesting solid waste A process and apparatus for reducing the volume and mass of solid waste (A) by initially subjecting the solid waste to a digestive enzymatic solution (20) agitated by fluid jets (28) under conditions which convert substrate (12) into a liquid waste which ... | 07/05/1994 |
| 5320960 | Method of preparing solution enriched in xylanase using low molecular weight alcohol, organic salt and inorganic salt A method for preparing an aqueous solution enriched in xylanase from an aqueous mixture containing cellulase proteins and xylanase is disclosed. The method involves adding an amount of a low molecular weight alcohol to an aqueous mixture containing cellul... | 06/14/1994 |
| 5276141 | Process for purifying a highly glycosylated protein The invention proposes a process for purifying a highly glycosylated protein from a crude preparation which comprises the action (i) of adding to said preparation a divalent metal ion in a sufficient amount in order to form a mixture which precipitates an... | 01/04/1994 |
| 5234829 | Purification method for materials having nick translation ability The present invention pertains to a method of purification of DNA polymerase I, and the polymerase and Nick-translation activities thereof. In one embodiment, the method of purification is directed to circumstances where there are amplified amounts of the... | 08/10/1993 |
| 5208155 | D-amino acid oxidase and method for isolation thereof A Triqonopsis variabilis D-amino acid oxidase in substantially pure form and active against cephalosporin C is disclosed. This D-amino acid oxidase is isolated from Trigonopsis variabilis by a method which is performed in three steps, namely: (a) acidifyi... | 05/04/1993 |
| 5151358 | Processes for the recovery of naturally produced chymosin Disclosed are methods for the recovery and purification of naturally produced chymosin. In particular, disclosed are methods for the recovery and purification of chymosin from aqueous solutions containing chymosin, pepsin and other contaminants.... | 09/29/1992 |
| 5139943 | Processes for the recovery of microbially produced chymosin Disclosed are methods for the recovery of microbially produced chymosin. In particular, disclosed are methods for the recovery of chymosin from the fermentation beer arising from culturing microorganisms which have been engineered so as to produce chymosi... | 08/18/1992 |
| 5120650 | Method for producing crystalline glucose isomerase In a process for growing enzyme crystals, small crystals are continuously removed from a crystallizer, dissolved and returned to the crystallizer to maintain a supersaturated state. The method permits the growing of large crystalline enzymes of uniform si... | 06/09/1992 |
| 5112752 | Biocatalytic oxidation using soybean and other legume peroxidases Biocatalytic oxidative processes wherein oxidizable substrates are reacted with peroxides in the presence of a peroxidase such as soybean peroxidase or another legume peroxidase; an oxidative coupling reaction for producing phenolic resins; a method for t... | 05/12/1992 |
