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Patent No. 5500234

Crispy Chip Sandwich and Process of Producing a Sandwich Product

A food product comprising a multilayer cookie or snack having outer layers formed from a crispy type edible food product such as a potato chip or corn chip, etc. with an intermediate marshmallow layer being in contact with the inner surface of each crispy chip and one or more filler substances.

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Class 435/470 - Introduction via electroporation, particle, fiber or microprojectile mediated insertion, or injection


Subclass of Class 435 - Chemistry: molecular biology and microbiology
Definition: Processes wherein the nucleic acid molecule is introduced
No. of patents: 199
Last issue date: 09/23/2008


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NumberTitleIssue Date
7427503Method for transferring material in a cell system
Described is a method for method for transferring material through the membrane of at least one cell, wherein the transfer is carried out in the presence of trehalose. This method is applicable in particular in the field of genetic engineering and biotechnology....
09/23/2008
7407781Oocyte recording chamber
An oocyte recording chamber for electrophysiological measurements. The recording chamber includes a base and a cover attached to the base. The cover and the base define a chamber having a size sufficient to accommodate an oocyte. The recording chamber includes a fir...
08/05/2008
7351877Lambda integrase mediated recombination in plants
The present disclosure provides methods for obtaining the targeted integration of a DNA molecule into the genome of a host cell using a recombinase. The methods disclosed herein can be used with a variety of host cells, including, for example, dicotyledonous and mon...
04/01/2008
7344887Methods and products for delivering biological molecules to cells using multicomponent nanostructures
This invention is predicated on the present applicants' discovery that nanostructures comprising discrete regions of different composition can be used to deliver to a biological cell a desired combination of molecules in close proximity. Different molecules can be s...
03/18/2008
7341864Molecule transferring device, auxiliary for molecule transferring device, and molecule transferring method
A molecule introducing apparatus is provided with a power supply circuit 20 for supplying power, a step-up transformer 30 which is supplied with power from the power supply circuit 20 and which outputs a high voltage, a switching transistor 2...
03/11/2008
7335815Nucleic acid sequences to proteins involved in isoprenoid synthesis
Methods are provided for producing plants and seeds having altered isoprenoid content and compositions. The methods find particular use in increasing the isoprenoid levels in plants, and in providing desirable isoprenoid compositions in a host plant cell. ...
02/26/2008
7332339Transferring materials into cells using porous silicon
Porous and/or polycrystalline silicon are used in the delivery of substances into cells. The porous and/or polycrystalline silicon can be formed into micropiercers, microneedles and biolistic bullets for penetration of the cell. The control of the pore size and poro...
02/19/2008
7309822Inbred corn line G0302
Broadly this invention provides an invention which is inbred corn line G0302. The methods for producing a corn plant by crossing the inbred line G0302 are also encompassed by the invention. Additionally, the invention relates to the various parts of inbred G0302 inc...
12/18/2007
7300776L-amino acid-producing bacterium and a method for producing L-amino acid
An L-amino acid-producing strain of Escherichia coli is bred by modifying an Escherichia coli K12 strain or a derivative thereof so as to become resistant to L-valine and have an ability to produce one or more L-amino acids selected from the group cons...
11/27/2007
7297339mutants and vaccines comprising same
Mutant strains of Rhodococcus equi are disclosed, and vaccines comprising same. ...
11/20/2007
7294761Method for efficiently producing transgenic plant using auxin precursor
A method for producing a transgenic plant, which comprises: (A) introducing a vector into a plant cell, wherein the vector is a vector for gene introduction into a plant and comprises: a desired gene, and a selectable marker gene comprising a gene encoding an enzyme...
11/13/2007
7294758Materials and methods for producing geminivirus resistant plants
The subject invention pertains to materials and methods for producing plants that are resistant to infection by geminiviruses and other related viruses. Methods of the invention comprise transforming a plant with a polynucleotide wherein when the polynucleotide is e...
11/13/2007
7288645Nucleic acid molecules encoding sucrose transporters
This invention relates to an isolated nucleic acid fragment encoding a sucrose transport protein. The invention also relates to the construction of a chimeric gene encoding all or a portion of the sucrose transport protein, in sense or antisense orientation, wherein...
10/30/2007
7279322Electrospraying apparatus and method for coating particles
An electrospraying apparatus and/or method is used to coat particles. For example, a flow including at least one liquid suspension may be provided through at least one opening at a spray dispenser end. The flow includes at least particles and a coating material. A s...
10/09/2007
7276646Synthetic polynucleotide coding for human lactoferrin, vectors, cell and transgenic plants containing it
A synthetic polynucleotide encoding human lactoferrin, modified with respect to the natural gene so as to maximize its expression in vegetals, on the basis of the preferential use of the codons is described. Moreover, the vectors containing such sequence, that havin...
10/02/2007
7259016Shaken not stirred
This invention relates to a method of transforming plant cells using whiskers on various types of target tissue. And further relates to a method of transforming plant cells using whiskers with an improved agitation method. ...
08/21/2007
7211713Methods and compositions for the production of stably transformed, fertile monocot plants and cells thereof
This invention relates to a reproducible system for the production of stable, genetically transformed maize cells, and to methods of selecting cells that have been transformed. One method of selection disclosed employs the Streptomyces bar gene introduced by ...
05/01/2007
7205462Inbred corn line G2202
Broadly this invention provides an invention which is inbred corn line G2202. The methods for producing a corn plant by crossing the inbred line G2202 are also encompassed by the invention. Additionally, the invention relates to the various parts of inbred G2202 inc...
04/17/2007
7179601Methods of identifying plant disease-resistance genes
The invention relates to a method of identifying a plant disease-resistance gene that includes the steps of (a) providing a plant tissue sample; (b)introducing by biolistic transformation into the plant tissue sample a candidate plant disease-resistance gene; (c) ex...
02/20/2007
7176186Stimulation of cell-mediated immune responses by targeted particulate genetic immunization
The present invention relates to various methods of genetic immunization for the purpose of providing antigen-specific immunity in a mammalian host, including a human host. The invention is based on the ability to direct particulate polynucleotides which express an ...
02/13/2007
7166768Whisker-mediated transformation of embryogenic cotton suspension cultures
Embryogenic cotton suspension cultures can be transformed by elongated, needle-like structures called “whiskers”. The process comprises the agitation of cotton suspension cultures in the presence of DNA and whiskers, whereby DNA uptake and integration thereof is...
01/23/2007
7132289Method for introducing foreign matters into living cells
A method for introducing a foreign matter into a cell, includes the steps of placing a small particle carrying a foreign matter at a part of a cell surface of a living cell, boring a hole in a cell wall and/or a cell membrane by irradiating and treating said part of...
11/07/2006
7129394Transgenic tea through biolistic using leaf explants
The present invention relates to Production of transgenic tea (Camellia sinensis (L.) O. Kuntze) through biolistic. ...
10/31/2006
7122715In vitro method to create circular molecules for use in transformation
The present invention relates generally to transgenic plants. More specifically, it relates to methods and compositions for the introduction of DNA using circular molecules that are not able to replicate outside a host cell. The circular molecules contain site-speci...
10/17/2006
7109034Method for electro-permeabilization of individual cellular and organellar structures and use thereof
The invention relates to a method for permeabilization of a cell structure consisting of a single cell, an intracellular structure or an organelle comprising the following steps: (a) microelectrodes, preferably two carbon fiber electrodes or hollow fiber electrodes,...
09/19/2006
7101703Electrofusion microelectrode
The present invention is directed to an electrofusion microelectrode used in the alignment, manipulation, fusion, or electroporation of cells. This device is particularly useful for transplantation of cells and cellular components. ...
09/05/2006
7094606Use of mixed duplex oligonucleotides to effect localized genetic changes in plants
The invention concerns the use of duplex oligonucleotides about 25 to 30 base pairs to introduce site specific genetic alterations in plant cells. The oligonucleotides can be delivered by mechanical (biolistic) systems or by electrpoporation of plant protoplasts. Th...
08/22/2006
7067716Methods and compositions for the introduction of molecules into cells
The present invention is directed to the introduction of molecules, including nucleic acids, carbohydrates, plant growth regulators and peptides into cells and tissues. The present invention is also directed to media and methods for enhancing embryogenic callus prod...
06/27/2006
7030294Expression of trehalose 6-phosphate synthase and trehalose 6-phosphate phosphatase in plant plastids
The invention provides novel stress tolerant transgenic plants which express trehalose 6-phosphate synthase and trehalose-6-phosphate phosphatase in their plastids. ...
04/18/2006
7026286Methods and compositions for the introduction of molecules into cells
The present invention is directed to the introduction of molecules, including nucleic acids, carbohydrates, plant growth regulators and peptides into cells and tissues. The present invention is also directed to media and methods for enhancing embryogenic callus prod...
04/11/2006
7019116PRO 1387 polypeptides
The present invention is directed to novel polypeptides and to nucleic acid molecules encoding those polypeptides. Also provided herein are vectors and host cells comprising those nucleic acid sequences, chimeric polypeptide molecules comprising the polypeptides of ...
03/28/2006
7001754Methods and compositions for the introduction of molecules into cells
The present invention is directed to the introduction of molecules, including nucleic acids, carbohydrates, plant growth regulators and peptides into cells and tissues. The present invention is also directed to media and methods for enhancing embryogenic callus prod...
02/21/2006
6972197Plant chromosome compositions and methods
The present invention provides for the identification and cloning of functional plant centromeres in Arabidopsis. This will permit construction of stably inherited minichromosomes which can serve as vectors for the construction of transgenic plant and animal ...
12/06/2005
6951971Method of making water stress or salt stress tolerant transgenic cereal plants
The present invention relates to a method for conferring tolerance to salt stress and drought stress in a monocot plant including transforming the monocot plant with an expression cassette comprising at least one ABRC unit, a minimal promoter, and a DNA molecule tha...
10/04/2005
6946587Method for preparing fertile transgenic corn plants
Fertile transgenic Zea mays (corn) plants which stably express heterologous DNA which is heritable are disclosed along with a process for producing said plants. The process comprises the microjectile bombardment of friable embryogenic callus from the plant to...
09/20/2005
6929949Corona ion generating method and apparatus for the manipulation of molecules and biological cells
The present invention provides a method and apparatus adapted to facilitate the entry of a preselected molecule into the intracellular space of a cellular sample through the use of ions generated by a corona charge source. With the present method and apparatus, mole...
08/16/2005
6929950Microprojectile delivery system and particulate product
The invention relates to a particulate product comprising at least one microprojectile; characterized in that the or at least one of the microprojectiles comprises silicon. The invention also relates to devices and components used in the microprojectile implantation...
08/16/2005
6916656Non-linear amplitude dielectrophoresis waveform for cell fusion
An object of the invention is to provide a method of treating biological cells prior to subjecting the biological cells to cell fusion pulses which includes the step of treating the biological cells with pre-fusion electric field waveforms which change amplitude in ...
07/12/2005
6911586Garden bean named ‘210104’
A novel garden bean cultivar, designated ‘210104’, is disclosed. The invention relates to the seeds of garden bean cultivar ‘210104’, to the plants of garden bean line ‘210104’ and to methods for producing a bean plant by crossing the cultivar ‘210104...
06/28/2005
6881411Method of stimulating an immune response by administration of host organisms that express intimin alone or as a fusion protein with one or more other antigens
This invention satisfies needs in the art by providing intimin, the Enterohemorrhagic Escherichia coli (EHEC) adherence protein, alone or as a fusion protein with one or more other antigens, expressed by transgenic plants and the use of those plants as vehicl...
04/19/2005
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