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Class 435/469 - Introduction via Agrobacterium


Subclass of Class 435 - Chemistry: molecular biology and microbiology
Definition: Processes wherein the nucleic acid is introduced into the
No. of patents: 259
Last issue date: 12/13/2011


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NumberTitleIssue Date
8076142Rooted plant assay system
Disclosed herein are rooted plant assay systems, methods of making rooted plant assay systems, and methods of using rooted plant assay systems for high throughput screening of test polynucleotides for their ability to effect phenotypic traits having agricultural val...
12/13/2011
8030076Soybean transformation method
A method is disclosed for the Agrobacterium-mediated germline genetic transformation of soybean. The method is based on Agrobacterium-mediated gene delivery to individual cells in a freshly germinated soybean meristem, which cells can be induced direct...
10/04/2011
7939328Method of transforming monocotyledons using scutella of immature embryos
Disclosed is a method of transforming monocotyledons which necessitates only a short period from the transformation to the regeneration of a whole plant as compared with the conventional methods, thus reducing the frequency of occurrence of mutants, and can be gener...
05/10/2011
7682829Methods for corn transformation
The present invention relates to a novel transformation system for generating transformed corn plants. In particular, the invention relates to a rapid selection system at an elevated temperature that allows faster and more efficient transformation. ...
03/23/2010
7666677Production of stilbenes in plant hairy root cultures
Improved methods for production of stilbenoids including resveratrol, pinosylvin and their respective derivatives are provided, including producing hairy roots from plant cells and eliciting production of the stilbenes. The plant cells in an embodiment are infected ...
02/23/2010
7611898transformation of stolons
The invention provides methods for transforming monocotyledonous plants with Agrobacterium, for example, using stolons as a target tissue. The invention allows creation of transgenic plants without the need for callus as a target tissue for transformation, th...
11/03/2009
7572635Method for agrobacterium transformation for dihaploid corn plants
The present invention relates to a novel system for generating transformed dihaploid plants from haploid cells and tissues without the use of chromosome doubling agents. ...
08/11/2009
7449335Precise cutting
The present invention provides methods for enhancing plant transformation. One such method entails making an excision in a selected seedling at the point where two cotyledons meet, and then vortexing the cut seedling in a solution comprising a transforming bacterium...
11/11/2008
7368631Regeneration and genetic transformation of
The present invention is directed to a method of Acacia mangium regeneration through organogenesis and a method of genetic transformation of Acacia mangium. The method of regeneration comprises inducing callus from different parts of seedlings and vege...
05/06/2008
7364902Mobilization of viral genomes from T-DNA using site-specific recombination systems
The invention relates to methods and compositions for site-specific recombinase-mediated mobilization of viral replicons and associated DNAs of interest from T-DNA. The methods of the invention comprise Agrobacterium-mediated transfer of T-DNA to a plant cell...
04/29/2008
7351877Lambda integrase mediated recombination in plants
The present disclosure provides methods for obtaining the targeted integration of a DNA molecule into the genome of a host cell using a recombinase. The methods disclosed herein can be used with a variety of host cells, including, for example, dicotyledonous and mon...
04/01/2008
7303909Binary vectors for the improved transformation of plants systems
The present invention relates to a binary vector which is distinguished by the combination of a variety of elements, demonstrates particularly high compatibility from the cloning aspect and which, besides the T-DNA flanked by the right and left border, contains an a...
12/04/2007
7297838Doubled haploid production and genetic transformation
Development of an efficient and cost-effective doubled haploid production system and genetic transformation system are the prerequisite to initiate haploid breeding and genetic modification in flax respectively. Pre-culturing anthers on a high osmotic, high auxin an...
11/20/2007
7294758Materials and methods for producing geminivirus resistant plants
The subject invention pertains to materials and methods for producing plants that are resistant to infection by geminiviruses and other related viruses. Methods of the invention comprise transforming a plant with a polynucleotide wherein when the polynucleotide is e...
11/13/2007
7288645Nucleic acid molecules encoding sucrose transporters
This invention relates to an isolated nucleic acid fragment encoding a sucrose transport protein. The invention also relates to the construction of a chimeric gene encoding all or a portion of the sucrose transport protein, in sense or antisense orientation, wherein...
10/30/2007
7288694Methods for enhancing segregation of transgenes in plants and compositions thereof
The compositions and methods are provided that enhance the selection of transgenic plants having two T-DNA molecules integrated into a plant genome at different physical and genetic loci. The compositions are DNA constructs that comprise novel arrangements of T-DNA ...
10/30/2007
7288409Method of introducing a plurality of genes into plants
A method of concurrently introducing multiple genes to plants and trees is provided. The method includes an Agrobacterium-mediated gene delivery system by which multiple genes together with a single selectable marker gene are simultaneously transferred and in...
10/30/2007
7285705Plant transformation method
A transformation method comprising inoculation and co-cultivation of a target tissue, from a target plant, with Agrobacterium, at a time when the target tissue is in its natural plant environment, followed by generation of a transgenic plant via dedifferentia...
10/23/2007
7279336Methods and compositions for enhanced plant cell transformation
Methods and compositions to increase Agrobacterium transformation efficiency (frequencies) in both dicot and monocot host plants include adding histones to the host plant at most transiently, and using histones and L-cysteine at certain stages in monocot tran...
10/09/2007
7276374Enhanced plant cell transformation by addition of host genes involved in T-DNA integration
Adding at least one gene involved in plant host cell T-DNA integration enhances transformation by Agrobacterium. The histone H2A gene encoded by the Arabidopsis RAT5 gene increases transformation frequencies of plants, most likely by causing overexpres...
10/02/2007
7276646Synthetic polynucleotide coding for human lactoferrin, vectors, cell and transgenic plants containing it
A synthetic polynucleotide encoding human lactoferrin, modified with respect to the natural gene so as to maximize its expression in vegetals, on the basis of the preferential use of the codons is described. Moreover, the vectors containing such sequence, that havin...
10/02/2007
7259292Methods of producing transgenic gramineae plants having resistance to iron deficiency
A method for producing transgenic gramineae plants having tolerance to iron deficiency by transforming the plants with a barley gene encoding nicotianamine aminotransferase (NAAT) and transgenic gramineae plants are provided. The present invention also provides tran...
08/21/2007
7253337Gene regulatory region that promotes early seed-specific transcription
Nucleic acid sequence capable of regulating transcription during embryogenesis in plants is provided. This sequence may be used in transgenic plants to promote high levels of expression of foreign and endogenous genes in developing seeds to affect seed lipid metabol...
08/07/2007
7238862Efficiency -mediated wheat transformation method
The present invention relates to an improved transformation and regeneration system for wheat. In particular, the invention relates to enhancements in the glyphosate selection system. The transformation method is efficient and reliable for production of fertile plan...
07/03/2007
7214860Methods of using non-plant encoding nucleic acids
Methods of creating and enhancing insect resistance in plants by introducing non-plant lipases into plants are provided. Plants with enhanced insect resistance and seed from plants thereof are provided. DNA sequences encoding insecticidal lipases and insecticidal li...
05/08/2007
7205150Transgenic plants over-expressing plant ADP-glucose pyrophosphatase
A method for obtaining a transgenic plant that over-expresses a soluble isoform AGPPase enzyme. The method includes a step of transforming a plant with a vector comprising a polynucleotide of SEQ ID NO: 7 linked to a promoter that promotes expression of the polynucl...
04/17/2007
7193128Methods for generating or increasing revenues from crops
The present invention provides methods of doing business and providing services. For example, methods of increasing the revenue of crops are provided. To this end, the method includes the use of a nucleic acid sequences of plant centromeres. This will permit constru...
03/20/2007
7193134Flax (L.) fatty acid desaturase
The present invention is directed to promoters of flax conlinin and ω-3 desaturase genes. The promoters guide high levels of the expression exclusively in flax developing seeds. This specific expression pattern concomitant with the biosynthesis of storage lipids an...
03/20/2007
7157620Enhanced transformation and regeneration of transformed embryogenic pine tissue
The present invention relates to methods for the transformation and regeneration of transformed embryogenic tissue of coniferous plants. In particular, the invention relates to improved methods for transforming embryogenic tissue of coniferous plants and for regener...
01/02/2007
7145058Efficient method of preventing growth of microbial genetic transformant after transformation
The present invention relates to an efficient and cost effective method of preventing growth of genetic transformant bacteria Agrobacterium tumefaciens after transformation in plants by using tea leaf extract as a bactericide, wherein said method leads to eli...
12/05/2006
7126041High efficiency gene targeting in plants
The present invention provides a method for the targeted insertion of a nucleotide of interest into a specific chromosomal site within a plant cell. The method comprises the steps of: (a) providing a plant cell, the plant cell optionally but preferably having a hete...
10/24/2006
7122716Enhanced plant cell transformation by addition of host genes involved in T-DNA integration
Adding at least one gene involved in plant host cell T-DNA integration enhances transformation by Agrobacterium. The histone H2A gene encoded by the Arabidopsis RAT5 gene increases transformation frequencies of plants, most likely by causing overexpres...
10/17/2006
7112721Methods and constructs for plant transformation
The present invention relates to novel methods for the transformation of crop species, novel methods for the selection and identification of transformed plant cells and novel methods for recovery of regenerated whole plants. The method also relates to the developmen...
09/26/2006
7067719Transformation of sp. with using embryogenic callus cultures
The present invention relates to a method for transforming Allium species with a heterologous gene using Agrobacterium. ...
06/27/2006
7060876Method for transforming monocotyledons
The invention relates to a method for transforming a monocotyledonous plant. The time required from transformation to regeneration of a plant is shorter using the inventive method so that the frequency of emergence of mutants is smaller than the conventional methods...
06/13/2006
7057090Agrobacterium-mediated transformation of turfgrass
A method of obtaining transgenic turfgrass plants by an Agrobacterium-mediated transformation protocol is disclosed. The protocol makes use of a modified Agrobacterium vector system in which selectable marker genes and other genes of interest are opera...
06/06/2006
7045357Efficiency agrobacterium-mediated plant transformation method
The present invention relates to a rapid transformation and regeneration system for plants. In particular, the invention relates to a plant tissue preparation system. The transformation method is efficient and reliable for production of fertile plants with improved ...
05/16/2006
7029908Plasmids for plant transformation and method for using the same
The invention provides novel Ti-plasmid vectors for Agrobacterium-medicated plant cell transformation. Plamsids of the invention are constructed with a DNA sequence (ie., a negative selectable maker or a DNA sequence that inhibits DNA unwinding) flanking the ...
04/18/2006
7026528Methods for the production of stably-transformed, fertile wheat employing agrobacterium-mediated transformation and compositions derived therefrom
Disclosed are processes for producing stably transformed fertile wheat a system of transforming wheat via Agrobacterium. This invention provides methods transforming a variety of explants, such as freshly isolated or pre-cultured immature embryos, embryogenic...
04/11/2006
6989236Methods to identify plant metabolites
This invention provides materials and methods to manipulate the plant genome at the level of single plant cells in culture resulting in the ability to assign metabolic functionality to plant genes involved in the production of biologically active molecules and to cr...
01/24/2006
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