...that it was melting ice cream that inspired the invention of the outboard motor? It was a lovely August day and Ole Evinrude was rowing his boat to his favorite island picnic spot. As he rowed, he watched his ice cream melt and wished he had a faster way to get to the island. At that moment the idea for the outboard motor was born!
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| Number | Title | Issue Date |
| 7393685 | Method for cultivating cancer cells from human tissue and device for preparing tissue samples The invention relates to a method for cultivating cancer cells for scientific serial assays, wherein a tissue sample which is heterogeneous with respect to contaminants, normal cells and tumor cells is locally separated in a sequential-parallel splitting method. The... | 07/01/2008 |
| 7300794 | Accelerated culture system for intestinal epithelial cell monolayers The present invention provides a unique medium for the cultivation of intestinal cell lines. The medium allows for the development of a highly differentiated intestinal epithelial cell monolayer in a much shorter period of time than currently possible without the ai... | 11/27/2007 |
| 7255879 | Post-partum mammalian placenta, its use and placental stem cells therefrom The present invention provides a method of extracting and recovering embryonic-like stem cells, including, but not limited to pluripotent or multipotent stem cells, from an exsanguinated human placenta. A placenta is treated to remove residual umbilical cord blood b... | 08/14/2007 |
| 7253334 | Methods for cloning non-human mammals using reprogrammed donor chromatin or donor cells The invention provides methods for cloning mammals that allow the donor chromosomes or donor cells to be reprogrammed prior to insertion into an enucleated oocyte. The invention also features methods of inserting chromosomes or nuclei into recipient cells. ... | 08/07/2007 |
| 7132279 | Epidermal melanocyte culture formulations The present invention provides methods and compositions for providing graft recipients with epidermal melanocytes. Specifically, the methods and compositions of the invention provide for populations of epidermal melanocytes that may be isolated from a patient and cu... | 11/07/2006 |
| 7118746 | Conditioned cell culture medium compositions and methods of use Novel products comprising conditioned cell culture medium compositions and methods of use are described. The conditioned cell medium compositions of the invention may be comprised of any known defined or undefined medium and may be conditioned using any eukaryotic c... | 10/10/2006 |
| 7060496 | Culture medium for in vitro culture of in vitro-produced porcine embryo and method for in vitro production of porcine embryo using the culture medium The invention provides an in vitro culture medium for in vitro-produced porcine embryo for the in vitro culture thereof, which can improve the quality of the resulting blastocyst and can raise the ratio of the development into fetus and infant after transfer, along ... | 06/13/2006 |
| 7045148 | Method of collecting placental stem cells A method of collecting embryonic-like stem cells from a placenta which has been treated to remove residual cord blood by perfusing the drained placenta with an anticoagulant solution to flush out residual cells, collecting the residual cells and perfusion liquid fro... | 05/16/2006 |
| 6964869 | Method and composition for skin grafts A chimeric skin comprising immortalized human keratinocyte cells cocultured with donor keratinocytes is disclosed. ... | 11/15/2005 |
| 6943023 | Insect cell primary culture medium, extra cellular matrix, and process of preparing an insect culture cell line in a short period of time using the medium and matrix A novel cell culture medium suitable for primary culture of insect cells, an insect-derived water-soluble chitin, and a process of preparing an insect culture cell line in a short period of time by using the insect primary culture medium and the insect-derived water... | 09/13/2005 |
| 6878546 | Product and method for swelling a cell nucleus without DNA replication The present invention concerns products and methods particularly useful for activating and analyzing non-dividing cell nuclei. The featured products include activating egg extracts, cytostatic factor (CSF) extracts, kits containing these extracts, and a microchamber... | 04/12/2005 |
| 6861255 | Method of using fish plasma components for tissue culture A process of using a fish plasma component as a nutrient medium component for tissue culture includes obtaining blood from a fish that is a progeny of domesticated broodstock that are reared under consistent and reproducible conditions, separating plasma from the bl... | 03/01/2005 |
| 6838284 | Cell culture medium containing growth factors and L-glutamine The invention relates to a culture medium for culturing cells, in particular human cells in a process for tissue engineering bone. The medium comprises glucose, a mineral, a vitamin, a growth factor and L-glutamine, wherein the L-glutamine is present in a concentrat... | 01/04/2005 |
| 6803209 | Preparation for use in cell culture in which an interfering compound is absent or depleted A cell culture preparation and methods are provided which are suitable for production in cells of a first protein in a class of proteins where the medium is deficient in a second protein in a related class, where the second protein is normally present in the serum a... | 10/12/2004 |
| 6790659 | In vitro production of amoebocytes from tachypleus gigas in leibovitz culture medium The present invention provides a process for large scale in vitro production of amoebocytes of Indian Horseshoe Crab (Tachypleus gigas) (T. gigas) from dissected gill flaps of T. gigas, in Leibovitz L-15 culture medium concentration (2×)... | 09/14/2004 |
| 6743629 | Culture medium for in vitro culture of in vitro-produced porcine embryo and method for in vitro production of porcine embryo using the culture medium The present invention provides methods for in vitro culturing of an in vitro produced porcine embryo by culturing the embryo in a medium containing lactate and pyruvate without glucose and then in a medium containing glucose without lactate and pyruvate. ... | 06/01/2004 |
| 6709864 | Adipogenic differentiation of human mesenchymal stem cells A composition which comprises human mesenchymal stem cells which have the potential to differentiate into cells of more than one connective tissue type and a composition which induces cells from the mesenchymal stem cell population to differentiate into the adipogen... | 03/23/2004 |
| 6682695 | Methods for sterilizing biological materials by multiple rates Methods are disclosed for sterilizing biological materials to reduce the level of one or more biological contaminants or pathogens therein, such as viruses, bacteria (including inter- and intracellular bacteria, such as mycoplasmas, ureaplasmas, nanobacte... | 01/27/2004 |
| 6599740 | Method of using fish plasma components for tissue culture A process of using a fish plasma component as a nutrient medium component for tissue culture includes obtaining blood from a fish, separating plasma from the blood, and extracting one or more specific components of the plasma. The tissue is cultured using... | 07/29/2003 |
| 6562621 | Method of using fish ovarian fluid for culture and preservation of mammalian cells A method of using fish ovarian fluid for culture and preservation of mammalian cells includes obtaining ovarian fluid from a fish, and culturing mammalian cells in media including a commercially defined medium and a nutrient medium, wherein the nutrient m... | 05/13/2003 |
| 6514711 | Immortalized human keratinocyte cell line A method for testing the effects of various factors on human skin equivalent is disclosed. The method involves providing a human stratified squamous epithelial cell culture of an immortalized human keratinocyte cell line that forms a reconstructed epiderm... | 02/04/2003 |
| 6500668 | Culture medium for avian embryonic cells A culture medium for avian embryonic cells and an avian cell culture medium is disclosed. The culture medium is characterized in that it has elements complementary to avian embryonic cells. The complementary elements are cytokines, fibroblast growth facto... | 12/31/2002 |
| 6495135 | Immortalized human keratinocyte cell line A spontaneously immortalized human keratinocyte cell line is disclosed. In a preferred embodiment, this cell line is ATCC 12191. In another embodiment of the invention, a method of assaying the effect of a test tumor cell modulation agent is disclosed. Th... | 12/17/2002 |
| 6485724 | Immortalized human keratinocyte cell line A spontaneously immortalized human kerazinocyte cell line is disclosed. In a preferred embodiment, this cell line is ATCC 12191. In another embodiment of the invention, a method of assaying the effect of a test tumor cell modulation agent is disclosed. Th... | 11/26/2002 |
| 6426219 | Nitric oxide-scavenging system for culturing oocytes, embryos, or other cells Nitric oxide adversely affects survival and development of cells such as oocytes and embryos in vitro, particularly in a co-culture system. The addition of a nitric oxide inhibitor such as hemoglobin to such systems eliminates this toxic effect, and promo... | 07/30/2002 |
| 6383805 | Epithelial cell cultures for in vitro testing A method and a medium for culturing epithelial cells of both normal and malignant origin is provided. The method entails physically disaggregating tissue samples, placing the resulting fragments onto a surface comprised of basement membrane matrix compone... | 05/07/2002 |
| 6376238 | Culture media for neurons, methods for preparing the culture media, and methods for culturing neurons The present invention is directed to a culture medium for neurons prepared by adding albumin to a culture supernatant obtained from a culture of primary astroglial cells in a trophic medium supplemented with insulin and transferrin. The culture medium of ... | 04/23/2002 |
| 6255109 | Nitric oxide-scavenging system for culturing oocytes, embryos, or other cells Nitric oxide adversely affects survival and development of cells such as oocytes and embryos in vitro, particularly in a co-culture system. The addition of a nitric oxide inhibitor such as hemoglobin to such systems eliminates this toxic effect, and promo... | 07/03/2001 |
| 6245567 | Activating egg extracts and method of preparation The present invention concerns products and methods particularly useful for activating and analyzing non-dividing cell nuclei. The featured products include activating egg extracts, cytostatic factor (CSF) extracts, kits containing these extracts, and a m... | 06/12/2001 |
| 6228640 | Programmable antigen presenting cell of CD34 lineage A method is described whereby a dendritic cell derived from the CD34 hematopoietic cell lineage is directed to become a super antigen presenting cell that has been pulsed with tumor cell RNA or RNA expression products. The protocol provides for programmin... | 05/08/2001 |
| 6214567 | Immortalized human keratinocyte cell line A spontaneously immortalized human keratinocyte cell line is disclosed. In a preferred embodiment, this cell line is ATCC 12191. In another embodiment of the invention, a method of assaying the effect of a test tumor cell modulation agent is disclosed. Th... | 04/10/2001 |
| 6146890 | Method and system for cultivating macrophages A method of cultivating macrophages from blood, including collecting a quantity of blood, fractionating the quantity of blood into a plasma fraction, a white blood cell fraction generally including monocytes, and a red blood cell fraction, segregating the... | 11/14/2000 |
| 6130086 | Composition for an in vitro fertilization medium The present invention aims to provide a medium composition for in vitro fertilization, in particular, a composition usable in the culture of ova or early embryos which are fertilized eggs, the preparation or culture of sperm, and the pre-treatment of ova ... | 10/10/2000 |
| 6114168 | Active retinoic acid-free culture medium for chicken embryonic stem cells A culture medium for culturing chicken embryonic stem (ES) cells is disclosed. The culture medium is used for supporting avian ES cell cultures. The components of the avian ES cell media include cytokines, fibroblast growth factors, insulin-like growth fa... | 09/05/2000 |
| 6103523 | Pluripotent rabbit cell lines and method of making The present invention relates to a rabbit embryonic stem (ES) cell line, comprising at least 70%, preferably 80 to 90% undifferentiated cells and obtainable by isolating the inner cell mass of 5.5 days postcoitus blastocysts and culturing them on feeder c... | 08/15/2000 |
| 6087174 | Growth medium for primary pancreatic tumor cell culture The present invention provides a method of treating cancer comprising (a) obtaining a tumor cell line, (b) modifying the tumor cell line to render it capable of producing an increased level of a cytokine relative to the unmodified tumor cell line, and (c)... | 07/11/2000 |
| 6074874 | Epithelial cell cultures for in vitro testing A method and a medium for culturing epithelial cells of both normal and malignant origin is provided. The method entails physically disaggregating tissue samples, placing the resulting fragments onto a surface comprised of basement membrane matrix compone... | 06/13/2000 |
| 6054317 | System for the cell culture and cryopreservation of marine invertebrates A system for the culture of eukaryotic cells of marine invertebrates employing a culture medium containing a mixture of sodium, magnesium, chlorine, potassium, calcium, bromine, and sulfate is disclosed, as well as a system for the cryopreservation of suc... | 04/25/2000 |
| 5916809 | Medium for culturing normal human epidermal melanocytes Provided is a medium for culturing normal human epidermal melanocytes in vitro. The medium comprises a basal medium for culturing animal cells, Ca2+ at a final concentration of between about 0.15 mM and about 1.2 mM and Mg2+ at a f... | 06/29/1999 |
| 5912177 | Stem cell immobilization Haematopoietic stem cells (which term includes early progenitor cells) are immobilized on a substrate coated with a fibrin matrix and including a substance capable of both binding to the fibrin matrix and also having an RGD amino acid sequence for binding... | 06/15/1999 |