Class 435/359 - Expressing recombinant tPA

Subclass of Class 435 - Chemistry: molecular biology and microbiology
Definition: Subject matter wherein the Chinese Hamster ovary cell expresses
No. of patents: 37
Last issue date: 09/09/2008

Number	Title	Issue Date
7423135	β-actin and rpS21 promoters and uses thereof The invention relates to isolation of novel β-actin and ribosomal protein S21 (rpS21) promoters and uses thereof. In particular, this invention features nucleotide sequences for rodent β-actin promoters including, hamster, rat, and mouse, and hamster rpS21 promote...	09/09/2008
7280680	Method and apparatus for observing three-dimensional localizations of in vivo expressed genes as well as method and apparatus for observing minute three-dimensional localizations of in vivo expressed genes In order to make observation of localizations of in vivo expressed genes possible in an entire individual living organism such as animals, and plants, a living genetic recombinant specimen containing a marker that can be detected at the time when specific genes are ...	10/09/2007
RE39792	Method for culturing Chinese hamster ovary cells A biochemically defined culture medium for culturing engineered Chinese hamster ovary (CHO) cell lines, which is essentially free from protein, lipid and carbohydrate isolated from an animal source, having water, an osmolality regulator, a buffer, an energy source, ...	08/21/2007
7166445	Methods and host cells for improved cell culture The invention provides improved methods of recombinant protein production in cell culture. More specifically, the invention relates to the modulation of the IGF-1 signaling pathway in cells so as to improve production characteristics. ...	01/23/2007
7091004	Regulation of translation for recombinant protein production The invention provides cells that produce increased levels of recombinant protein by modulating the activity of translational regulator gene products that are downstream targets of PKB alpha, methods of making such cells, and methods of using such cells. Such transl...	08/15/2006
6667172	Cell and tissue culture modeling device and apparatus and method of using same A cell and tissue culture modeling device comprising a housing having an interior chamber, an inlet port in fluid communication with the internal chamber, an outlet port in fluid communication with the internal chamber, a plurality of hollow fibers dispos...	12/23/2003
6506598	Cell culture process A glycoprotein is produced by a process comprising culturing mammalian host cells expressing nucleic acid encoding said glycoprotein in the presence of (a) a factor that modifies growth state in a cell culture, (b) a divalent metal cation that can adopt a...	01/14/2003
6413744	Methods and host cells for improved cell culture The invention provides improved methods of recombinant protein production in cell culture. More specifically, the invention relates to the modulation of the IGF-1 signaling pathway in cells so as to improve production characteristics....	07/02/2002
5985607	Recombinant DNA molecules and expression vectors for tissue plasminogen activator A recombinant DNA molecule adapted for transfection of a host cell comprising a nucleic acid molecule encoding mammalian erythropoietin or tissue plasminogen activator, an expression control sequence operatively linked thereto and at least one SAR element...	11/16/1999
5849574	Human tPA production using vectors coding for DHFR protein A method for producing tissue plasminogen activator (t-PA) in eukaryotic host cells is disclosed. Enhanced levels of t-PA production are obtained by co-amplification of the t-PA gene through treatment of cultures transformed with mutant or wild type DHFR ...	12/15/1998
5763253	Methods of preparing tissue plasiminogen activator derivative composition Tissue plasminogen activator (t-PA) derivatives are produced in useful quantities using recombinant DNA techniques. Specific derivatives include amino acid deletion derivatives and amino acid substitution derivatives. A deletion derivative lacking the N-t...	06/09/1998
5753486	Human tissue plasminogen activator A human tissue plasminogen activator (t-PA) is produced in useful quantities using recombinant DNA techniques. The invention disclosed thus enables the production of t-PA free of contaminants with which it is ordinarily associated in its native cellular e...	05/19/1998
5736135	Method for making variant secreted proteins with altered properties A screening method for the selection of mutagenized proteins that are normally secreted by cells is described. The method includes the development of a cloning vector for the expression of secretory proteins as fusion proteins on the cell surface of trans...	04/07/1998
5728565	Methods of preparing tissue plasminogen activator derivatives Tissue plasminogen activator (t-PA) derivatives are produced in useful quantities using recombinant DNA techniques. Specific derivatives include amino acid deletion derivatives and amino acid substitution derivatives. A deletion derivative lacking the N-t...	03/17/1998
5658788	Compounds and methods for treatment of thromboembolic disorders The present invention provides derivatives of tissue plasminogen activator that lack the Finger, Growth Factor and Kringle 1 domains and comprise a Kringle 2 domain that is monoglycosylated at a site other than that of t-PA. Using recombinant DNA techniqu...	08/19/1997
5658759	Recombinant DNA expression vectors The invention provides expression vectors containing the promoter, enhancer and substantially complete 5'-untranslated region including the first intron of the major immediate early gene of human cytomegalovirus. Further vectors including the hCMV-MIE DNA...	08/19/1997
5646009	Hybrid vector and method resulting in protein overproduction by eukaryotic cells A hybrid vector carrying a first and second DNA segments operationally linked thereto, the first DNA segment encoding a protein capable of cross-linking to the cap structure of mRNA and mediating ribosome-binding, and the second DNA segment encoding a pol...	07/08/1997
5641655	Methods for producing thrombopoietin polypeptides using a mammalian tissue plasminogen activator secretory peptide DNA constructs useful in the production of thrombopoietin are disclosed. In general, the DNA constructs comprise a first DNA segment encoding a fusion of an amino-terminal secretory peptide joined to a thrombopoietin polypeptide and one or more additional...	06/24/1997
5616486	Tissue plasminogen activator having zymogenic or fibrin specific properties Tissue plasminogen activator (t-PA) zymogens and variants are prepared, including a fibrinolytically active variant of t-PA that has an amino acid alteration at a site within the protease domain of t-PA as compared with the corresponding wild-type t-PA, w...	04/01/1997
5614190	Tissue plasminogen activator having zymogenic or fibrin specific properties Tissue plasminogen activator (t-PA) zymogens and variants are prepared, including a fibrinolytically active variant of t-PA that has an amino acid alteration at a site within the protease domain of t-PA as compared with the corresponding wild-type t-PA, w...	03/25/1997
5510248	Stable recombinant meizothrombin-like polypeptides An isolated polynucleotide which encodes human prothrombin is modified to encode amino acid substitutions at positions 155, 271 and 284 of the prothrombin polypeptide. A second polynucleotide is further modified to encode an additional amino acid substitu...	04/23/1996
5439679	Peptide plasminogen activators Novel polypeptide compositions based on the amino acid sequence of tissue plasminogen activator (tPA) are provided having improved properties over natural tissue plasminogen activator. Particularly, enhanced specific activity, reduced response to inhibiti...	08/08/1995
5407819	Human plasminogen activator variants having amino acids 37-42 substituted and a method for their manufacture A TPA variant with replacement of amino acids is provided. In a typical embodiment, the variant has an amino acid sequence with replacement of the amino acid group consisting of asparagine at the 37th position, serine at the 38th position, glycine at the ...	04/18/1995
5346824	DNA encoding variants of tissue plasminogen activators and expression vectors and hosts thereof A fibrinolytically active amino acid sequence variant of a plasminogen activator is prepared that has one or more glycosylation sites in regions that are not glycosylated in the native molecule. DNA sequences can be prepared that encode the variants, as w...	09/13/1994
5342775	Plasminogen activator A novel plasminogen activator which is identical in peptide sequence to naturally occurring human prourokinase except that the 155th amino acid counting from the N-terminal amino acid (serine)] is other than the 155th amino acid (proline) of naturally occ...	08/30/1994
5270198	DNA molecules encoding variants of tissue plasminogen activators, vectors, and host cells A fibrinolytically active amino acid sequence variant of a plasminogen activator is prepared that has one or more glycosylation sites in regions that are not glycosylated in the native molecule. DNA sequences can be prepared that encode the variants, as w...	12/14/1993
5262170	Tissue plasminogen activator having zymogenic or fibrin specific properties and substituted at amino acid positions 296-299, DNA molecules encoding them, vectors, and host cells Tissue plasminogen activator (t-PA) zymogens and variants are prepared, including a fibrinolytically active variant of t-PA that has an amino acid alteration at a site within the protease domain of t-PA as compared with the corresponding wild-type t-PA, w...	11/16/1993
5246850	DNA molecules encoding human tissue plasminogen activator variants with decreased clearance, vectors, and host cells Biologically active tissue plasminogen activator (t-PA) variants with decreased clearance as compared to wild-type t-PA are prepared, including variants that have one or more amino acid alterations in at least the kringle-1 and/or kringle-2 domain(s) of t...	09/21/1993
5244806	DNA encoding novel tissue plasminogen activator derivatives having kringles 1 and 2 deleted, vectors and host cells The invention discloses modified forms of the enzyme tissue plasminogen activator (t-PA) in which recombinant DNA techniques are utilized to modify the structure of the t-PA cDNA to express a modified t-PA which retains the ability to activate plasminogen...	09/14/1993
5242819	DNA molecules encoding hybrid proteins of tissue plasminogen activator and urokinase Novel single-chain hybrid plasminogen activators having an amino acid sequence composed of at least two subsequences corresponding in amino acid identity and number to subsequences of human t-PA and of human u-PA, and mutants thereof in which at least one...	09/07/1993
5219738	Animal cell transformant, method for obtaining the transformant and method for producing desired foreign gene product by using the transformant By screening an animal cell transformant, which has been obtained by introducing into wild-type animal cells a wild-type DHFR gene and a structural gene which codes a desired useful substance, with a medium containing methotrexate (MTX) at a concentration...	06/15/1993
5147643	DNA sequences encoding human t-PA substituted at position 275 or at positions 275 and 277 and pharmaceutical compositions Biologically active mutant tissue plasminogen activators are disclosed wherein site directed mutagenesis, for example, of a two-chain activation site renders the mutants resistant to conversion to the two-chain form....	09/15/1992
5122458	Use of a bGH gDNA polyadenylation signal in expression of non-bGH polypeptides in higher eukaryotic cells The present invention relates to the use of the polyadenylation signal from the gene for bovine growth hormone to achieve a high level of expression of peptides in eukaryotic cells. Accordingly, the present invention provides a recombinant DNA compound co...	06/16/1992
5106741	Tissue plasminogen activator (TPA) analogs This invention discloses human tissue plasminogen activator (TPA) analogs. The analogs are TPA-like molecules that have had the native domain regions either rearranged, delected, added or a combination thereof. The analogs are the product of the expressio...	04/21/1992
5087572	DNA encoding human plasminogen modified at the cleavage site A cleavage-resistant plasminogen molecule is provided that is conveniently produced in recombinant cells by expression of a nucleic acid sequence encoding the plasminogen molecule. Preferably the plasminogen is a sequence variant with a modification in it...	02/11/1992
5010002	Human t-PA production using vectors coding DHFR protein A method for producing tissue plasminogen activator (t-PA) in eukaryotic host cells is disclosed. Enhanced levels of t-PA production are obtained by co-amplification of the t-PA gene through treatment of cultures transformed with mutant or wild type DHFR ...	04/23/1991
4853330	Human tissue plasminogen activator Human tissue plasminogen activator (t-PA) is produced in useful quantities using recombinant DNA techniques. The invention disclosed thus enables the production of t-PA free of contaminants with which it is ordinarily associated in its native cellular env...	08/01/1989

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Class 435/359 - Expressing recombinant tPA