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Class 800/294 - Via Agrobacterium


Subclass of Class 800 - Multicellular living organisms and unmodified parts thereof and related processes
Definition: Method wherein the polynucleotide is introduced into the
No. of patents: 395
Last issue date: 04/03/2012


1                    
NumberTitleIssue Date
8148609Uses for Yerba Santa
Methods of in vitro propagation of plants of the genus Eriodictyon are described, including, in particular embodiments, plants of the species E. californicum, E. trichocalyx and E. sessilifolium. Methods of producing transgenic plants of the gen...
04/03/2012
8148608Systems and methods for clonal expression in plants
The present invention provides systems and methods for generating clonal root lines, clonal root cell lines, clonal plant cell lines, and clonal plants and for expressing gene products in such cell lines and plants. In some embodiments, a viral vector containing a p...
04/03/2012
8143484Method for regulating agrobacterium-mediated transformation
A method for regulating expression of a virulence gene of Agrobacterium is described. The method comprises the steps of stimulating cereal cells, such as sorghum, so as to produce an active, typically phenolic, compound and exposing the Agrobacterium t...
03/27/2012
8115061Plant transformation method
The invention provides a transformation method comprising inoculation and co-cultivation of a target tissue, from a target plant, with Agrobacterium, at a time when the target tissue is in its natural plant environment, followed by generation of a transgenic ...
02/14/2012
8106258Use of a low-oxygen environment in plant transformation
Use of a low-oxygen environment before the transformation process can enhance transformation of plants. Low oxygen during regeneration can also increase the culturability of recalcitrant genotypes. ...
01/31/2012
8101823Method of controlling a cellular process in a multi-cellular organism
A method of controlling a genetically-modified multi-cellular organism or a part thereof, comprising the following steps: (a) providing a multi-cellular organism or a part thereof, whereby cells of said multi-cellular organism or said part contain a heterologous nuc...
01/24/2012
8101824Method of constructing transgenic ice plant
The present invention provides a method of transforming common ice plant by gene transfer using a microorganism belonging to the genus Agrobacterium and a method of producing a transformed common ice plant. The present invention also provides a stable transfo...
01/24/2012
8093458Biologically safe transient protein expression in plants
A process of producing a protein of interest by expression of said protein of interest from a sequence of interest in a plant or in plant leaves, comprising: (a) transfecting said plant or said plant leaves by infiltrating said plant or said plant leaves with an ...
01/10/2012
8076537Method of breeding germinable transgenic broadleaved tree species
Methods of breeding germinable transgenic broadleaved tree species are disclosed which include the following steps: (1) the main stem of a tree is cutoff at the height of 50-160 cm above the ground; (2) the transection is dipped with 0.3-0.5 OD600 of a su...
12/13/2011
8067673Methods for plant regeneration, transformation and production of insect resistant transgenic Okra
The present description concerns methods for regeneration of whole plant from the explants obtained from the Abelmoschus species preferably A. esculentus. In addition the present description also concerns methods for transforming okra plant, plant cell...
11/29/2011
8058514Agrobacterium-mediated method for producing transformed plant without selection step
A novel Agrobacterium-mediated method for producing a transformed monocotyledonous plant is provided. The transformation method involves (i) a coculture step for culturing an Agrobacterium-inoculated monocotyledonous plant tissue with a coculture mediu...
11/15/2011
8053638Method for agrobacterium-mediated transformation of plants
The invention provides methods and compositions for enhancing the efficiency of Agrobacterium-mediated transformation of host cells such as plant cells. Plant expression constructs comprising a gene encoding a VIP2 or VIP2-like polypeptide are provided, as we...
11/08/2011
8049067Organogenic transformation and regeneration
This invention provides methods of transforming organogenic plant cells and regenerating plants from transformed cells. ...
11/01/2011
8030544Methods for plant transformation using spectinomycin selection
The present invention relates to methods and compositions for transforming soybean, corn, cotton, or canola explants using spectinomycin as a selective agent for transformation of the explants. The method may further comprise treatment of the explants with cytokinin...
10/04/2011
8017837Method for producing transgenic plants
A method for producing transgenic plants, including treating a target tissue using plasmolyzing media (PM) which contains 4% to 10% of sucrose and 100 μM to 300 μM of Acetosyringone (AS) and gold particles. The target tissue is infected by a bacterial suspension u...
09/13/2011
7994399Methods for the production of stably transformed, fertile plants
The present invention relates to improved methods for the incorporation of DNA into the genome of a Zea mays plant by means of Agrobacterium-mediated transformation. Preferred is the use of the Zea may lines deposited with American Type Culture ...
08/09/2011
7989678Disarmed strains, Ri-plasmids, and methods of transformation based thereon
The present invention relates “disarmed” strain variants of Agrobacterium strain K599 (NCPPB 2659), “disarmed” plasmid variants of the Ri-plasmid pRi2659, and derivatives thereof, and methods employing these strains and plasmids in plant transformatio...
08/02/2011
7960611Method for promoting efficiency of gene introduction into plant cells
A method for gene introduction by which higher efficiency for gene introduction than that by the conventional Agrobacterium method may be attained simply and without injuring the tissue is disclosed. According to the method of the present invention, the effic...
06/14/2011
7960610Methods for enhancing segregation of transgenes in plants
The compositions and methods are provided that enhance the selection of transgenic plants having two T-DNA molecules integrated into a plant genome at different physical and genetic loci. The compositions are DNA constructs that comprise novel arrangements of T-DNA ...
06/14/2011
7928291Use of multiple transformation enhancer sequences to improve plant transformation efficiency
The present invention relates to methods and compositions for improving the efficiency of Agrobacterium- and Rhizobium-mediated plant cell transformation by use of additional transformation enhancer sequences, such as overdrive or TSS sequences, operab...
04/19/2011
7928292Refined plant transformation
The present invention provides methods for producing transgenic plants based on an optimized transfer of DNA from Agrobacterium to plant cells, and/or on an optimized integration of the transferred DNAs into plant cell genomes. It also provides Agrobacteri...
04/19/2011
7910803Transformation in
The present invention relates to plant biotechnology and specifically to a method for genetically transforming Camelina sativa with Agrobacterium-mediated transformation system. It comprises Camelina sativa for producing homologous and heterolog...
03/22/2011
7902426Method of improving gene transfer efficiency into plant cells utilizing heat and centrifugation
A method for gene transfer by which higher efficiency for gene transfer than that by the conventional Agrobacterium method may be attained simply and without injuring the tissue is disclosed. According to the method of the present invention, the efficiency of...
03/08/2011
7897838Methods for high efficiency transformation and regeneration of plant suspension cultures
The present invention provides methods for high efficiency plant transformation via Agrobacterium-mediated T-DNA conjugation to suspension-cultured cells or calli. The methods described herein employ membranes or filters as porous solid support for the co-cul...
03/01/2011
7893323Transformation of . with agrobacterium using embryogenic callus cultures
The present invention relates to a method for transforming Allium species with a heterologous gene using Agrobacterium. ...
02/22/2011
7855325Use of nitric oxide modulators in -mediated plant transformation
Use of nitric oxide modulators during the transformation process can enhance Agrobacterium-mediated transformation of plants. ...
12/21/2010
7812222Method of transducing gene into plant material
The present invention relates to a method of introducing a gene into plant material via Agrobacterium. A method of the present invention is characterized in that it comprises: 1) pressurizing a plant material, and then 2) infecting the plant material with an ...
10/12/2010
7803988Plant transformation method
The invention provides a transformation method comprising inoculation and co-cultivation of a target tissue, from a target plant, with Agrobacterium, at a time when the target tissue is in its natural plant environment, followed by generation of a transgenic ...
09/28/2010
7754944Polynucleotide constructs
Synthetic modular polynucleotide constructs are useful for gene expression in plants, methods for making and using such constructs, and plants transformed with such constructs. The constructs comprise unique restriction sites for each modular element and may further...
07/13/2010
7737328Transformation and regeneration of plants
The invention relates to a novel transformation method for plants of the genus Allium. Plants transformed by the method are also provided. The method preferably involves an Agrobacterium tumefaciens-mediated transformation, involving the transformation...
06/15/2010
7714191Transformation of sp. with using embryogenic callus cultures
The present invention relates to a method for transforming Allium species with a heterologous gene using Agrobacterium. ...
05/11/2010
7709699Use of duckweed in high throughput screening
Methods for high-throughput screening in duckweed are disclosed. In one aspect, these methods are used to identify nucleotide sequences encoding polypeptides of interest. In another aspect, these methods are used to identify nucleotide sequences that modulate the ex...
05/04/2010
7709700Method for improving plant transformation efficiency by adding copper ion
The present invention provides a method for Agrobacterium-mediated gene introduction into a plant material, comprising: 1) treating the plant material, and then 2) infecting the plant material with an Agr...
05/04/2010
7692063Production of foreign nucleic acids and polypeptides in sprout systems
The present invention provides systems and methods for producing a nucleic acid or protein in transgenic sprouted seedlings or sprouted seedlings engineered to transiently express a nucleic acid or protein of interest. The sprouted seedlings of the invention are gro...
04/06/2010
7659447Increasing host plant susceptibility to agrobacterium infection by overexpression of the arabidopsis VIP1 gene
The present invention is directed to methods and compositions which employ an Arabidopsis VIP1 gene or VIP1-like gene from another plant species for purposes of increasing host plant susceptibility to Agrobacterium infection. Methods for increasing sus...
02/09/2010
7619138Precise breeding
The present invention relates to a new plant breeding process. The process improves the agronomic performance of crop plants by using genetic material that is also used in classical breeding. Instead of sexually recombining entire genomes at random, as is done in cl...
11/17/2009
7608758Plant system for comprehensive gene function analysis with the use of full-length cDNA
A gene causative of a phenotype is easily specified and thus gene functions are comprehensively analyzed. A method for analyzing a gene function includes (a) infecting a plant group with a full-length cDNA library containing an expression regulatory sequence to intr...
10/27/2009
7598430Refined plant transformation
The present invention provides methods for producing transgenic plants based on an optimized transfer of DNA from Agrobacterium to plant cells, and/or on an optimized integration of the transferred DNAs into plant cell genomes. It also provides Agrobacteri...
10/06/2009
7592508High-efficiency -mediated transformation of cotton using petiole explants
A method is disclosed for producing a transgenic cotton plant by Agrobacterium-mediated transformation of petiole tissue. The method comprises the steps of (a) obtaining cotton petiole explants, (b) exposing the petiole explants to a culture of Agrobacteri...
09/22/2009
7569746Method for delivery of proteins to plant cells
The invention relates to the use of a modified Agrobacterium to deliver proteins directly to plant cells. Proteins of interest are delivered to the plant host in the form of a fusion protein with the Agrobacterium virulence protein VirF. Nucleotide seq...
08/04/2009
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