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| Number | Title | Issue Date |
| 6869934 | Method of purifying calcium ion-binding protein The present invention relates to a method for purifying a calcium ion-binding protein by cation exchange chromatography. The present invention provide a method for isolating and purifying a calcium ion-binding protein in a simple and efficient manner from a liquid s... | 03/22/2005 |
| 6620917 | Method for the purification and aqueous fiber spinning of spider silks and other structural proteins Methods are described for the purification and spinning of recombinant and non-recombinant proteins. Specifically, the lysis of bacteria and purification of silk proteins occur in a single solution of organic acid. Bacterial proteins are hydrolyzed while ... | 09/16/2003 |
| 6562951 | Method of solubilization and naturation of somatotropins A method for the solubilization and/or naturation of a somatotropin involves contacting a somatotropin with a detergent composition and water under conditions effective to obtain a naturated somatotropin, wherein the detergent composition may be a C1... | 05/13/2003 |
| 6410694 | Method for solubilization and naturation of somatotropins A method for the solubilization and/or naturation of a somatotropin involves contacting a somatotropin with a detergent composition and water under conditions effective to obtain a naturated somatotropin, wherein the detergent composition may be a C1... | 06/25/2002 |
| 6335430 | Process of producing polyphenolic adhesive proteins and proteins produced in accordance with the process The invention relates to a process of producing MAP-products from mussel feet. The process is characterised by extracting the mussel feet in a weakly acid aqueous solution containing, for instance, 1-10 percent by weight of a weak acid and 0.5-3 percent b... | 01/01/2002 |
| 6326029 | Resorbable extracellular matrix for reconstruction of cartilage tissue A resorbable extracellular matrix for reconstruction of cartilage tissue, the matrix being substantially free from non-native collagen, and including a purified collagen II material formed form natural cartilage and having fibers of native collagen II whi... | 12/04/2001 |
| 6043216 | Methods and composition for detecting and treating kidney diseases associated with adhesion of crystals to kidney cells An autocrine crystal adhesion inhibitor called CAI is an anionic, sialic acid-containing glycoprotein secreted by kidney epithelial cells that blocks adhesion of calcium oxalate monohydrate (COM) crystals to the cell surface. Novel amino acid sequences ar... | 03/28/2000 |
| 6034224 | Method for solubilization and naturation of somatotropins A method for the solubilization and/or naturation of a somatotropin involves contacting a somatotropin with a detergent composition and water under conditions effective to obtain a naturated somatotropin, wherein the detergent composition may be a C1... | 03/07/2000 |
| 5998589 | Method of purifying human factor VIII The invention is a method of purifying Factor VIII comprising the steps of contacting a solution containing Factor VIII with a Factor VIII-binding substrate under conditions sufficient to bind Factor VIII to the substrate, wherein the Factor VIII-binding ... | 12/07/1999 |
| 5886157 | Expression and purification of human cytochrome P450 The invention provides a nucleic acid encoding a human cytochrome P450 2E1 comprising a 5' terminal deletion of 63 nucleotides, thereby encoding methionine at the first codon position of the 5' terminus, the codon ATG in the second codon position of the 5... | 03/23/1999 |
| 5840849 | Blends of collagen type I and collagen type III for food casings Blends of collagen type I and collagen type III having crosslinks of the pyridinoline (Pyrid), dihydroxylysinonorleucine (DHLNL) and histidinohyroxylysinonorleucine (HHL) types, wherein the amounts of the crosslinks satisfy the ratio: (Pyrid+DHLNL)/HHL=0.... | 11/24/1998 |
| 5610284 | Method of purification of human BCDF There are disclosed (i) a purification process for obtaining a human BCDF having the intramolecular disulfide linkage and the stereostructure of natural type human BCDF which comprises subjecting to an oxidation reaction and a refolding treatment a reduce... | 03/11/1997 |
| 5530100 | Methods for purification of recombinantly produced proteins Recovery of the 52/48 kDa tryptic fragment of vWF or peptide subfragments thereof produced in the form of inclusion bodies from recombinant host cells is carried out by providing a washed recombinant host cell suspension, adding a detergent and subjecting... | 06/25/1996 |
| 5367054 | Large-scale purification of egg immunoglobulin The present invention relates to methods for the isolation and purification of immunoglobulins or fragments thereof or other biologically active factors from non-immune or immune egg yolk, by extracting the yolk with a composition containing one or more m... | 11/22/1994 |
| 5324823 | Adsorbent for cellular fibronectin and a method for fractional purification of fibronectin The invention provides a selective adsorbent for cellular fibronectin (cFN) and a method for fractional purification of FN which includes contacting an FN material containing plasma fibronectin (pFN) and cFN with a crosslinked polysaccharide sulfate and/o... | 06/28/1994 |
| 5258497 | Process for purifying annexines The present invention relates to processes for purifying annexines. The overall process includes the steps of preparing a homogenized cell preparation and adjusting the pH to about 8.0 to 10.0, adding at least one bivalent cation, adding a phospholipid, w... | 11/02/1993 |
| 5245012 | Method to achieve solubilization of spider silk proteins A recombinant spider silk protein can be obtained in a commercially useful form by cloning and the expression in a host cell of a polynucleotide encoding an endogenous spider silk protein or variant thereof. The sequencing of a spider silk protein is made... | 09/14/1993 |
| 5071958 | Composition inducing a binding A composition for use in inducing binding between parts of mineralized tissue by regeneration of mineralized tissue on at least one of the parts, containing as an active constituent a protein fraction originating from a precursor to dental enamel, so call... | 12/10/1991 |
| 4894444 | Recovery and purification of immunogens from immunogen-alum complexes Immunologically intact protein or peptide immunogens are recovered from vaccines consisting of immunogen-aluminum hydroxide (alum) complexes. Recovery consists of dissolution of the complexes with an alkali metal salt of a carboxylic acid at a basic pH, r... | 01/16/1990 |
| 4873317 | Method of separating or purifying proteins and other biopolymers A method for the separation or purification of biopolymers comprises adsorbing the biopolymer on the surface of liquid oil droplets; and separating the adsorbed biopolymer from the oil droplets. The adsorbed biopolymer is separated from the oil droplets b... | 10/10/1989 |
| 4743583 | Sustained release protein compositions and method for making Lewis acid-Lewis base high molecular weight salt microparticulate material of the type referred to in U.S. Pat. No. 3,959,457, which include biologically active peptides and proteins solubilized in a non-aqueous, non-denaturing manufacturing solvent.... | 05/10/1988 |
| 4647455 | Process for extracting atrial natriuretic factor Diuretic and natriuretic extracts, at least partially characterized as peptides, have been obtained by homogenization of mammalian heart atria with an aqueous solution of a lower carboxylic acid. After precipitation of impurities by pH adjustment, the ext... | 03/03/1987 |
| 4530785 | Process for the purification of microbial protein isolates Substances producing an undesired odor and taste can be removed from microbial protein materials if these materials are treated with aqueous solutions which contain a phosphate and/or a hydrophilic low molecular weight aliphatic compound which contains at... | 07/23/1985 |
| 4482482 | Cesium trifluoroacetate solutions for the isolation of nucleic acids A method for simultaneously isolating DNA, RNA and/or protein fractions from a crude biological mixture containing DNA, RNA and/or protein fractions is disclosed. The method comprises adding the crude biological mixture to an aqueous solution of cesium tr... | 11/13/1984 |
| 4464295 | Simple and rapid method for extraction of proteins from bacteria 44A simple, rapid method for extracting proteins from pathogenic and nonpathogenic bacteria is described.... | 08/07/1984 |
| 4427782 | Isolation of principal outer membrane protein and antigen of Chlamydia trachomatis Procedures are presented for isolating the major outer membrane protein of Chlamydia trachomatis. The isolated protein is a species specific antigen which comprises about 60% of the C. trachomatis cell outer membrane structure. The protein has a molecular... | 01/24/1984 |
| 4357323 | Polysaccharide RBS substance and antitumor agent containing same A polysaccharide RBS substance having the ultraviolet absorption spectrum depicted in FIG. 1 and the infrared absorption spectrum depicited in FIG. 2 is obtained from rice bran by extraction with hot water. These substances have been tested for anti-tumor... | 11/02/1982 |
| 4325866 | Protein, PP11, a process for its preparation and its use What are disclosed are a new protein, PP11, obtainable from placental tissue, methods for isolating the protein in concentrated form, methods for making an antiserum to said protein, and methods for using said antiserum for monitoring pregnancy... | 04/20/1982 |
| 4294753 | Bone morphogenetic protein process The process of separating bone morphogenetic protein (BMP) from bone tissue comprising the steps of demineralizing bone tissue; treating the demineralized bone tissue under aqueous conditions with a water soluble neutral salt and a solubilizing agent for ... | 10/13/1981 |
| 4271147 | Process for the isolation of membrane proteins from Neisseria meningitidis and vaccines containing same Membrane proteins are isolated from Neisseria meningitidis by direct treatment of the cells with a detergent. The membrane proteins can be worked up into a vaccine.... | 06/02/1981 |
| 4267101 | Process for obtaining human relaxin from fetal membranes Human Relaxin is recovered from human fetal membranes, and preferably from the chorion-decidua membranes.... | 05/12/1981 |
| 4244973 | Process for producing a detoxified rapeseed protein concentrate A process for the detoxification of rapeseed which involves (a) autolyzing an aqueous mixture of rapeseed meal in the presence of myrosinase and ascorbic acid to achieve hydrolysis of glucosinolates present in the meal and (b) extracting the toxic product... | 01/13/1981 |
| 4239498 | Method of preparing thymic factors and composition A process for obtaining factors having immunological properties from thymus glands including aseptically collecting thymus glands from freshly slaughtered neo-natal vertebrates, comminuting the glands in an isotonic aqueous solution, removing fat and soli... | 12/16/1980 |
| 4080262 | Stabilizing agent for enzymes Enzymes, particularly labile enzymes, and most particularly microorganism derived labile enzymes, are stabilized by adding thereto a stabilizing agent comprising a protein fraction obtained by extracting yeast with water at 80° to 100° C., mixing the cl... | 03/21/1978 |
| 4042575 | Extraction of glycoproteins and sialic acid from whey Process for the treatment of dairy or casein factory whey, notably for the extraction of glycoproteins and sialic acid. The process includes ultrafiltration of casein whey, thermal flocculation of the proteins of the ultrafiltration retentate, phosphotungstic ... | 08/16/1977 |
| 4029641 | Process for the simultaneous obtention of insulin and pancreatin from swine pancreases A process for the simultaneous obtention of insulin and pancreatin from fresh or deep frozen swine pancreases by extracting the glands at a pH of about 5 to 7 with a reaction mixture containing at least 40% of acetone, separating the extracted substance a... | 06/14/1977 |
| 4016260 | Novel polypeptide produced by pseudomonas A novel polypeptide designated as Gamba A having anti-tumor activity, is produced by cultivating a variant strain of Pseudomonas cruciviae, i.e., Pseudomonas sp. No. 2205 (FERM-P 2132 ATCC 31155), and recovering the polypeptide from the cultured broth the... | 04/05/1977 |
| 3975343 | Solubilization of protein with ethanol-acetonitril-water solvent system A solvent system composed of ethanol-acetonitrile-water dissolved certain membrane proteins of biological origin. The solvent system was optically transparent to ultraviolet radiation from 200 to 400 nanometers (nm). This transparency permitted ascertainm... | 08/17/1976 |
