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| Number | Title | Issue Date |
| 7919318 | Process for isolating vascular endothelial cells from embryoid bodies differentiated from embryonc stem cells The present invention provides a process for isolating vascular endothelial cells from embryoid bodies differentiated from embryonic stem cells, which comprises: (a) treating embryoid bodies differentiated from embryonic stem cells with 0.005-0.015% trypsin and 0.05... | 04/05/2011 |
| 7413734 | Treatment of retinitis pigmentosa with human umbilical cord cells Cells derived from postpartum umbilicus and placenta are disclosed. Pharmaceutical compositions, devices and methods for the regeneration or repair of ocular tissue using the postpartum-derived cells are also disclosed. ... | 08/19/2008 |
| 7326572 | Endoderm cells from human embryonic stem cells This disclosure provides a system for producing pancreatic islet cells from embryonic stem cells. Differentiation is initiated towards endoderm cells, and focused using reagents that promote emergence of islet precursors and mature insulin-secreting cells. High qual... | 02/05/2008 |
| 7320872 | Ependymal neural stem cells and method for their isolation The invention relates to an ependymal neural CNS stem cell, which cell expresses the surface protein Notch 1 together with at least one surface protein chosen from the group of Notch 2, Notch 3, CAR (transmembrane protein binding adenovirus) and CFTR cystic fibrosis... | 01/22/2008 |
| 7109028 | Methods of isolating hepatic progenitors This invention relates to methods of isolating hepatoblasts utilizing panning techniques and fluorescence activated cell sorting. This invention further relates to isolated hepatoblasts and to a method of treating liver dysfunction as well as to methods of forming a... | 09/19/2006 |
| 7067496 | Methods for introducing genes into mammalian subjects Methods to obtain genetic modifications of cells in histoculture are described. Modification is assisted by treating the histoculture with collagenase prior to contacting the histoculture with the delivery vehicle for the desired gene. Hair follicles and other organ... | 06/27/2006 |
| 7045349 | Method of islet isolation using process control Advanced Islet Separation Technology incorporates an automated method, automated control methodology, process control interface, and automated apparatus to separate (isolate) and process pancreatic islets in a tissue suspension in physiologic process solution, utili... | 05/16/2006 |
| 7033831 | Islet cells from human embryonic stem cells This disclosure provides a system for producing pancreatic islet cells from embryonic stem cells. Differentiation is initiated towards endoderm cells, and focused using reagents that promote emergence of islet precursors and mature insulin-secreting cells. High qual... | 04/25/2006 |
| 7022520 | Cell culture media for mammalian cells A chemically defined mammalian cell culture medium is provided that supports maintenance and long term clonal growth of mammalian hepatocytes and other cells. ... | 04/04/2006 |
| 6946293 | Progenitor cells, methods and uses related thereto The present invention relates to a substantially pure population of viable pancreatic progenitor cells, and methods for isolating such cells. The present invention further concerns certain therapeutic uses for such progenitor cells, and their progeny. ... | 09/20/2005 |
| 6900051 | Process for obtaining mammalian insulin secreting cells in vitro and their uses The present invention relates to a process for obtaining mammalian insulin secreting cells in vitro, characterized in that it contains the following steps: a) preparation of the mammalian pancreatic tissues by removal of a pancreas, b) dissociation of the pancreatic... | 05/31/2005 |
| 6833270 | Apparatus and method for isolating cells from organs An apparatus and method for extracting cells from organs in which a digestion chamber includes at least one inlet and at least one outlet, and a separator for retaining the organ and permitting the cells and the physiologically compatible medium to exit the outlet. ... | 12/21/2004 |
| 6825027 | Method of production of purified hepatitis a virus particles and vaccine preparation The present invention provides methods of purification of Hepatitis A Virus from the supernatant of an infected cell culture and production of a preparation of purified HAV antigen. The present invention is also directed to an HAV vaccine composition comprising a pr... | 11/30/2004 |
| 6569442 | Preparation of polymer foam having gelled sol coating for intervertebral disc reformation Methods and materials are provided for reforming degenerated intervertebral discs of the spine of a vertebrate and in particular the spine of a human. Tissue is evacuated from the nucleus pulposus portion of a degenerated intervertebral disc space, and a ... | 05/27/2003 |
| 6537762 | Peptide mimotope to mycotoxin deoxynivalenol and uses thereof The present invention provides a peptide mimotope of the non-peptide mycotoxin deoxynivalenol. In particular, the peptide mimotope competes with deoxynivalenol for binding to a monoclonal antibody and is antagonistic to the inhibitory effects of deoxyniva... | 03/25/2003 |
| 6489164 | Isolation of cells from organ tissue using sonication A method for isolating specific viable cell types from surrounding organ tissue is provided. The method entails the use of sonication in conjunction with tissue dissociating agents to free the cells of interest. A specific application of the method is the... | 12/03/2002 |
| 6365405 | Compositions of chondrocytes, preparation and utilization This invention concerns compositions of chondrocytic cells, notably human ones, and the methods for preparing and using them. More specifically, the invention describes the production of autologous human chondrocyte suspensions, employing recombinant enzy... | 04/02/2002 |
| 6242252 | Hepatic progenitors and method of isolating same This invention relates to methods of isolating hepatic progenitors utilizing panning techniques and fluorescence activated cell sorting. This invention further relates to isolated hepatic progenitors and to a method of treating liver dysfunction as well a... | 06/05/2001 |
| 6238922 | Use of collagenase in the preparation of neural stem cell cultures The invention provides a method for using collagenase to dissociate neural stem cells in neural stem cell cultures when passaging aggregated neural stem cells. The collagenase treatment results in an increased cell viability and an increased number of pro... | 05/29/2001 |
| 6107543 | Culture of totipotent embryonic inner cells mass cells and production of bovine animals The use of totipotent embryonic stem cells to provide substantially identical cells for embryo cloning techniques is described. The method includes the culture of loose suspensions of inner cell mass cells of bovine animals to retrieve large populations o... | 08/22/2000 |
| 5994619 | Production of chimeric bovine or porcine animals using cultured inner cell mass cells Novel cultured inner cell mass (CICM) cells, and cell lines, derived from ungulates, in particular, pigs and cows, and methods for their preparation are provided. The subject CICMs possess similar morphology and express cell markers identically or substan... | 11/30/1999 |
| 5919703 | Preparation and storage of pancreatic islets A method, a solution and a chamber for the preparation and storage of pancreatic islets. The method includes contacting a pancreas with a warm collagenase solution, digesting the pancreas in the warm collagenase solution to form warm digest, adding cold p... | 07/06/1999 |
| 5905042 | Cultured inner cell mass cell lines derived from bovine or porcine embryos Novel cultured inner cell mass (CICM) cells, and cell lines, derived from ungulates, in particular, pigs and cows, and methods for their preparation are provided. The subject CICMs possess similar morphology and express cell markers identically or substan... | 05/18/1999 |
| 5879939 | Isolation of cells from organ tissue using sonication A method for isolating specific viable cell types from surrounding organ tissue is provided. The method entails the use of sonication in conjunction with tissue dissociating agents to free the cells of interest. A specific application of the method is the... | 03/09/1999 |
| 5855620 | Matrix substrate for a viable body tissue-derived prosthesis and method for making the same Disclosed is a process for treating body tissues such as vascular structures to remove barriers to recellularization before and after implantation in a human host. The body tissue, for example a heart valve, is subjected to a hypotonic/hypertonic treatmen... | 01/05/1999 |
| 5780301 | Serum-free medium for cultivation of postnatal central neurons Disclosed is a synthetic medium with PDGF, vitronectin, IL-1ଲ and BSA added to Eagle's minimum essential medium or medium with transferrin, insulin, progesterone and putrescine further added thereto. When cultivating the postnatal central neurons us... | 07/14/1998 |
| 5670358 | Method for inhibiting chymopapain and papain enzyme activity with polysaccharides of animal origin The activities of chymopapain or papain in enzyme mixtures are inhibited by the addition of glycogen, hyaluronic acid, or desulfated heparin. The process for isolating viable hepatocytes or pancreatic cells by treatment with papain or chymopapain is impro... | 09/23/1997 |
| 5556783 | Methods of culturing and modulating the growth of hair follicular stem cells A method of culturing hair follicular stem cells by isolating a subpopulation of follicular keratinocytes from the upper portion of a hair follicle, dispersing the isolated keratinocytes into a single cell suspension, and growing the dispersed isolated ke... | 09/17/1996 |
| 5424208 | Method for isolating cells from tissue with a composition containing collagenase and chymopapin Proteolytic enzyme compositions and processes for digesting connective tissue are provided. The enzyme compositions include collagenase, which is essentially free of toxins and non-collagen specific components, and chymopapain, which is essentially free o... | 06/13/1995 |
| 5348877 | Method of adapting anchorage-dependent cell lines to suspension conditions Normally anchorage-dependent insect cell lines are adapted to replicate under suspension conditions by addition of heparin to the culture medium and selection for resulting suspension-tolerant cells.... | 09/20/1994 |
| 5334527 | Epidermal graft system The present invention involves a readily produced skin autograft or silograft composite suitable for the supplementation or replacement of injured skin. A sheet of collagen-coated pliable material such as synthetic surgical dressing is used as a foundatio... | 08/02/1994 |
| 5326357 | Reconstituted cartridge tissue The present invention relates to cartilage tissue reconstituted on a substrate; to a method for producing reconstituted cartilage tissue; and to cartilage tissue reconstituted in vitro from isolated chondrocytes cultured on a substrate.... | 07/05/1994 |
| 5322790 | Islet isolation process A method of producing intact islets of Langerhans in an insulin producing condition uses a mixture of Hank's solution and 10% by volume fetal calf serum to ductally distend the human pancreas. The exocrine tissue of the pancreas is digested at about 37° ... | 06/21/1994 |
| 5079160 | Method to isolate clusters of cell subtypes from organs A method to obtain purified, well-defined cell populations from intact organs uses digestion of the distended organ with suitable proteolytic enzymes and harvest of the cell subpopulation by screening the effluent from treatment of the organ with physiolo... | 01/07/1992 |
| 4959319 | Process of corneal enhancement A process for establishing functional human corneal tissue consisting of an enhanced contact-inhibited endothelial monolayer derived from isolated human corneal endothelial cells with other integral corneal layers remaining intact. The process is divided ... | 09/25/1990 |
| 4937187 | Methods for separating malignant cells from clinical specimens Fragments of a biopsy sample on the order of about 50 to 5000 cells are preferred for establishing viable tumor cell cultures for purposes such as establishing cell lines, chemotherapeutic assays and the like. Such fragments retain the three-dimensional c... | 06/26/1990 |
| 4828991 | Tumor specific monoclonal antibodies Monoclonal antibodies produced by hybridoma or transformed B-cell lines deriveed from B-cells of cancer patients actively immunized with autologous tumor antigen. These monoclonal antibodies can be used in both diagnostic procedures and therapy for human ... | 05/09/1989 |
| 4820626 | Method of treating a synthetic or naturally occuring surface with microvascular endothelial cells, and the treated surface itself The present invention provides a novel method of treating a synthetic or naturally occurring implant, such as a vascular graft, intended for implantation in a human patient, comprising obtaining human microvascular rich tissue from that patient; separatin... | 04/11/1989 |
| 4786599 | Serum-free animal cell culture medium and methods for the primary culture and production of cell lines using this medium A serum-free animal tissue culture medium contains a mixture of six fatty acids and albumin or dextran. The medium is particularly adapted for the primary culture of rat liver epithelial cells and possibly in the presence of hormones and/or growth factors... | 11/22/1988 |
| 4769317 | Process for growing human epidermis, product thereof Procedures are disclosed for eliminating immune competent cells from a population of human epidermal cells. The cells free of immune competent cells are used for growing tissue sheets suitable for transplantation to recipients unrelated to the donor.... | 09/06/1988 |
