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| Number | Title | Issue Date |
| 8187879 | Collection and selection methods to isolate a stem cell population from human adult periodontal follicular tissues Methods for the isolation, expansion and storage of a population of stem cells belonging to human dental follicles, called FENC (Follicle-derived Embryonic Neural Crest stem cells,) including: a) Collection of the follicular sack in sterile conditions, digestion and... | 05/29/2012 |
| 8163554 | Intervertebral disc The present invention relates to an engineered biological material comprising or enriched for tissue of intervertebral disc; tissue derived from an engineered biological material; constructs comprising one or more tissues from an engineered biological material; meth... | 04/24/2012 |
| 8093051 | Method for isolation of inner cell mass and method of preparation of embryonic stem cell lines using inner cell mass isolated by the same A method for isolation of an inner cell mass and a method for preparation of embryonic stem cell lines using the inner cell mass isolated by the same. A blastocyst being free from a zona pellucida removed therefrom is placed on a feeder cell, and a micro cover glass... | 01/10/2012 |
| 8071379 | Methods of tissue engineering An improved substrate for growing mono-layers of adherent-type cells and methods of growing tissue structures, ex vivo. The improved substrate, which comprises a silicon substrate coated with a photo cleavable polymer, releases adherent cells non-enzymatically. Also... | 12/06/2011 |
| 8071380 | Skeletal muscle periangioblasts and cardiac mesangioblasts, method for isolation and uses thereof The present invention discloses the isolation and characterization of cells isolated either from adult skeletal muscle or from adult cardiac muscle. These cells are used for the treatment of muscular disorders including muscular dystrophy and cardiopathics, respecti... | 12/06/2011 |
| 8043854 | Method of decellularizing tissues In the transplant of a living organism tissue, such as a heart valve, taken from an animal, etc. into a human body, a cell removing solution for removing original cells from the living organism tissue is provided with flow approximately equal to the bloodstream of t... | 10/25/2011 |
| 7972851 | Liver specific chimeric regulatory sequence and use thereof The present invention relates to a chimeric regulatory sequence with liver cell specificity. The chimeric regulatory sequence includes a proximal regulatory sequence and a distal enhancer 5′ flanking region of human α-fetoprotein (AFP) gene. The chimeric regulato... | 07/05/2011 |
| 7939323 | Method of isolating cells from umbilical cord The present invention relates a method for isolating four cell types from a single umbilical cord as pure cultures. These cell lines (epithelial cells, fibroblasts, smooth muscle cells and endothelial cells) can be characterized and utilized in experimental models a... | 05/10/2011 |
| 7863045 | Isolation of skeletal precursor cells Cartilage-derived morphogenetic protein CDMP-1 or a transforming growth factor β having at least 80% homology with CDMP-1, or a factor co-expressed and/or co-detectable therewith, is used as a marker of skeletal precursor cells from any part of a mammalian body. | 01/04/2011 |
| 7846726 | Human fetal bladder-derived epithelial cells This invention discloses a substantially pure population of human urinary bladder-derived epithelial cells and methods of isolating and culturing the urinary bladder-derived epithelial cells. In addition, several uses of human urinary bladder-derived epithelial cell... | 12/07/2010 |
| 7829336 | Method for isolating a self-renewing, multipotent, slow-cycling cell The present invention relates to methods for isolating infrequently-dividing, slow-cycling cells, a feature which is typical of stem cells in their niche. The methods of the present invention are advantageously used as classical stem cells can be isolated. Further p... | 11/09/2010 |
| 7829337 | Cell-releasing agent and method of releasing cell sheet A cell-sheet releasing agent of the present invention contains an aminated polyrotaxane. The polyrotaxane constituting the skeleton of the cell-releasing agent of the present invention has a structure in which cavities of a plurality of cyclic molecules are threaded... | 11/09/2010 |
| 7745217 | Graft prosthesis devices containing renal capsule collagen The invention provides tissue graft compositions comprising collagen-based extracellular matrices derived from renal capsules of warm-blooded vertebrates. The invention further provides a process of harvesting and purifying a renal capsule to provide an extracellula... | 06/29/2010 |
| 7727764 | Non-isopycnic cell purification using percoll A method of “non-isopycnic” cell isolation and purification has the steps of adding a sample of blood to a defined volume to a corresponding ratio volume of EDTA solution to produce a volume of anti-coagulated blood; taking a predetermined volume of the anti-coa... | 06/01/2010 |
| 7723108 | Soft tissue processing The present invention is a process for preparing soft tissue such as tendons, ligaments, cartilage, fascia, dermis, human valves and human veins for implant in a human and removes cellular components and forms an decellular matrix having as major components collagen... | 05/25/2010 |
| 7695966 | Method for the separation and sorting of different biological objects utilizing differences in their viscoelastic properties A method for separating a multitude of biological objects by the type of the object. The method utilizes the differences in the viscoelastic properties for the different types of objects. As an example but not limited to: the method can be applied to most tumor cell... | 04/13/2010 |
| 7666675 | Nestin-expressing hair follicle stem cells Hair follicle stem cells are isolated from mammals by isolating nestin-expressing cells. These hair follicle stem cells are a source of adult stem cells for autologous or heterologous stem cell therapy. The stem cells can be systemically implanted into the mammal or... | 02/23/2010 |
| 7651856 | Mounting device for mounting a retainer means for a biological object and corresponding method for laser micro-dissection To enable contactless laser microdissection in a closed container (10, 11), for example in the form of a Petri dish, it is proposed according to the invention to use a holding device which comprises a receiving portion (4) for arrangement in the contai... | 01/26/2010 |
| 7553664 | Three-dimensional skin model The present invention relates to methods for cultivating dermal fibroblasts, methods for preparing in vitro dermis equivalents, methods for preparing three-dimensional in vitro skin equivalents, an in vitro dermis equivalent, a three-dimensional in vitro skin equiva... | 06/30/2009 |
| 7435586 | Method of purification of cells The present invention relates generally to a method for the generation of a substantially homogeneous population of undifferentiated cells. More particularly, the present invention relates to the purification of a substantially homogeneous population of stem cells a... | 10/14/2008 |
| 7413734 | Treatment of retinitis pigmentosa with human umbilical cord cells Cells derived from postpartum umbilicus and placenta are disclosed. Pharmaceutical compositions, devices and methods for the regeneration or repair of ocular tissue using the postpartum-derived cells are also disclosed. ... | 08/19/2008 |
| 7399632 | Mesenchymal precursor cell A method of enriching mesenchymal precursor sells including the step of enriching for cells based on at least two markers. The markers may be either i) the presence of markers specific for mesenchymal precursor cells, ii) the absence of markers specific for differen... | 07/15/2008 |
| 7393685 | Method for cultivating cancer cells from human tissue and device for preparing tissue samples The invention relates to a method for cultivating cancer cells for scientific serial assays, wherein a tissue sample which is heterogeneous with respect to contaminants, normal cells and tumor cells is locally separated in a sequential-parallel splitting method. The... | 07/01/2008 |
| 7374937 | Isolation and expansion of human marrow stromal cells The invention includes in vitro methods of inducing and enhancing proliferation of human marrow stromal cells for use in, for example, gene therapy and transplantation methods. The invention also includes a method of assessing the expandability (i.e., proliferative ... | 05/20/2008 |
| 7371576 | CD56 positive human adult pancreatic endocrine progenitor cells The invention relates to the discovery of a selective cell surface marker that permits the selection of a unique subset of pancreatic stems cells having a high propensity to differentiate into insulin producing cells or into insulin producing cell aggregates. ... | 05/13/2008 |
| 7368111 | Human antibodies specific for TGFβ2 Specific binding members comprising human antibody antigen binding domains specific for human transforming growth factor beta (TGFβ) bind specifically isoforms TGFβ2 and TGFβ1 or both, preferentially compared with TGFβ3. Specific binding members may be isolated ... | 05/06/2008 |
| 7361505 | Multipotent neural stem cell compositions The invention provides in vitro cell culture compositions consisting of neurospheres and culture medium, wherein the neurospheres consist of undifferentiated cells that are nestin+, glial fibrillary acid protein (GFAP)−, neurofilament (NF) | 04/22/2008 |
| 7338757 | Process for decellularizing soft-tissue engineered medical implants, and decellularized soft-tissue medical implants produced The invention provides methodologies and apparatus for producing acellular soft-tissue implants, both in small quantities and in commercializable quantities. Such soft-tissue implants include vascular graft substitutes. An acellular graft is produced by subjecting t... | 03/04/2008 |
| 7335811 | Method for collecting cells in M phase or Gphase and method for nuclear transplantation using the cells Methods for collecting cells in M phase or G1 phase, by which the percentage of M phase or G1 phase cells is higher than that attained by the conventional methods are disclosed. ... | 02/26/2008 |
| 7318998 | Tissue decellularization The present invention relates, in general, to tissue decellularization and, in particular to a method of treating tissues, for example, heart valves, tendons and ligaments, so as to render them acellular and thereby limit mineralization and/or immunoreactivity upon ... | 01/15/2008 |
| 7316926 | Immortalized vascular adventital cell line To provide an immortalized capillary pericyte line which maintains the original function/property of the cell line-deriving tissue, its establishment method, and the screening method for useful substance using the immortalized capillary pericyte line. Cerebral tissu... | 01/08/2008 |
| 7303912 | Cultures of GFAP nestin cells that differentiate to neurons Cultures of cells immunoreactive for glial fibrillary acidic protein (GFAP), as well as for the intermediate filament marker nestin were grown in a medium including epidermal growth factor (EGF) and serum. The cultured cells had the morphology of astroglial cells. T... | 12/04/2007 |
| 7288522 | Method of promoting erythropoiesis The present invention provides methods, compounds, pharmaceutical compositions, and kits for the augmentation of erythropoiesis by potentiating erythropoietin-induced differentiation with angiotensinogen, angiotensin I (AI), AI analogues, AI fragments and analogues ... | 10/30/2007 |
| 7282366 | Hepatocytes for therapy and drug screening made from embryonic stem cells It has been discovered that when pluripotent stem cells are cultured in the presence of a hepatocyte differentiation agent, a population of cells is derived that has a remarkably high proportion of cells with phenotypic characteristics of liver cells. In one example... | 10/16/2007 |
| 7279330 | Method for identifying modulating compounds of neurotransmitters A process for identifying compounds that can modulate the release of neuromediators is described in which, for example, at least one compound that is to be tested is brought into contact with a nerve tissue preparation and the possible modulating effect of the compo... | 10/09/2007 |
| 7279301 | Methods of determining deficiencies in intracellular levels on cysteine and glutathione The present invention provides a method for determining an antioxidant deficiency in a lymphocyte by determining the level of intracellular cysteine in the lymphocyte by measuring lymphocyte growth in a serum-free cell culture medium comprising cumene hydroperoxide ... | 10/09/2007 |
| 7273756 | Method for chondrocyte expansion with phenotype retention The present invention provides a method that maintains chondrocyte phenotype during serial expansion by culturing a population of chondrocytes in a defined serum-free culture medium containing cytokines and on a substrate that is modified by covalent attachment of h... | 09/25/2007 |
| 7264966 | Method of isolating cell or sample to be analyzed in cell There is disclosed a method of irradiating a specimen which is laid on a substrate and which includes a cell with a laser beam to remove an unnecessary specimen portion other than a cell portion from the substrate, next inserting the cell portion into one opening in... | 09/04/2007 |
| 7259138 | Anti-tumor agents A method for treating subjects with abnormal cell proliferation is provided. The method involves administering to subjects in need of such treatment an effective amount of an agent of Formula I, to inhibit cell proliferation such as that associated with tumor growth... | 08/21/2007 |
| 7252941 | Expression profiling based on histocultures Methods of obtaining faithful expression libraries from tissue samples comprise extraction of RNA from intact tissue cultured in three-dimensional sponge-gel based histocultures. ... | 08/07/2007 |
