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| Number | Title | Issue Date |
| 8153413 | High alkaline protease and use thereof The invention aims to provide a novel alkaline protease having peculiar properties such as high alkali activity, resistance to surfactants and calcium-dependent thermostability and exhibiting excellent performance in highly alkaline detergents, and a gene coding for... | 04/10/2012 |
| 7919300 | Glucose isomerase mutants This invention describes a series of recombinant Thermoanaerobacterium saccharolyticum glucose isomerases having improved catalytic activity and thermostability. The recombinant glucose isomerases can be used for direct production of fructose syrup containing... | 04/05/2011 |
| 7704719 | Glucose isomerase mutants, DNA thereof and use thereof This invention provides a series of recombinant Thermoanaerobacterium saccharolyticum glucose isomerases with improved catalytic activity and thermostability obtained by using recombinant techniques. These recombinant glucose isomerases comprise amino acid va... | 04/27/2010 |
| 7279319 | Method and apparatus for injecting oxygen into fermentation processes A sparger for delivering fluid comprising a sparger pipe for carrying the fluid from a fluid source and downwardly directed nozzles to direct the delivery of fluid is disclosed. A substantially vertical connection pipe connects the sparger to the nozzles. Also, a me... | 10/09/2007 |
| 7125695 | Directed evolution of microorganisms The present invention provides methods for directing the evolution of microorganisms comprising the use of mutator genes and growth under conditions of selective pressure. The method discloses mutator genes which can be used in the methods of the present invention a... | 10/24/2006 |
| 7087728 | Carbohydrate crosslinked glycoprotein crystals The present invention relates to the field of carbohydrate crosslinked glycoprotein crystals. Advantageously, such crosslinked glycoprotein crystals display stability to harsh environmental conditions, while maintaining the structural and functional integrity of the... | 08/08/2006 |
| 6927282 | Clarification of protein precipitate suspensions using anionic polymeric flocculants Protein suspensions comprising soluble and insoluble components are purified via flocculation with anionic polymers, such as polyacrylamides, potato starch, or modified cellulose. The procedure improves the efficiency of solid/liquid separations and can minimize or ... | 08/09/2005 |
| 6921651 | Process for the preparation of amino acids by using coryneform bacteria with attenuated 1-phosphofructokinase activity The invention relates to a process for the preparation of L-amino acids. The process includes fermenting the coryneform bacteria producing the desired L-amino acid, in which at least the gene coding for 6-phosphofructokinase and/or the gene coding for 1-phosphofruct... | 07/26/2005 |
| 6743619 | Nucleic acids and polypeptides The present invention provides novel nucleic acids, novel polypeptide sequences encoded by these nucleic acids and uses thereof. ... | 06/01/2004 |
| 6706503 | Directed evolution of microorganisms The present invention provides methods for directing the evolution of microorganisms comprising the use of mutator genes and growth under conditions of selective pressure. The method discloses mutator genes which can be used in the methods of the present invention a... | 03/16/2004 |
| 6680190 | Gene encoding phosphoglucoisomerase The present invention relates, in general, to a method of producing L-amino acids comprising culturing altered bacterial cells having increased amounts of NADPH as compared to unaltered bacterial cells whereby L-amino acids yields from said altered bacter... | 01/20/2004 |
| 6465238 | Gene encoding phosphoglucoisomerase The present invention relates, in general, to a method of producing L-amino acids comprising culturing altered bacterial cells having increased amounts of NADPH as compared to unaltered bacterial cells whereby L-amino acids yields from said altered bacter... | 10/15/2002 |
| 6410012 | Antimicrobial mediated bacterial DNA delivery This invention pertains in part to a method for delivering functional DNA or antigens. The desired DNA is introduced into attenuated bacteria able to enter cells. Once the bacteria is in the cell, antimicrobial agents are introduced such that they enter t... | 06/25/2002 |
| 6171856 | Methods and compositions relating to no-mediated cytotoxicity The present invention relates to methods and compositions for the treatment of diabetes involving free radicals. In particular, the present invention is directed to the treatment or prophylactic intervention of diabetes. The present invention demonstrates... | 01/09/2001 |
| 5945278 | Method and system for enhanced production of commercially important exoproteins in gram-positive bacteria The invention provides a method and expression system for enhancing secretion of hyperproduced homologous and heterologous exoproteins in gram-positive bacteria such as Bacillus sp. The method and system comprise overproduction of PrsA protein in a gram-p... | 08/31/1999 |
| 5900364 | Cross-linked glucose isomerase The invention relates to a novel water-insoluble glucose isomerase which is formed by a crystalline enzyme converted to solid form by cross-linking. The invention also concerns a process for the preparation of the novel crystalline glucose isomerase by cr... | 05/04/1999 |
| 5811280 | Cross-linked glucose isomerase The invention relates to a novel water-insoluble glucose isomerase which is formed by a crystalline enzyme converted to solid form by cross-linking. The invention also concerns a process for the preparation of the novel crystalline glucose isomerase by cr... | 09/22/1998 |
| 5710032 | Secondary-metabolite biosynthesis genes from actinomycetes, method of isolating them and their use Secondary-metabolite biosynthesis genes from actinomycetes, method of isolating them, and their use. The invention concerns secondary-metabolite biosynthesis genes from actinomycetes, a method of isolating secondary-metabolite and, in particular, 6-deoxy-... | 01/20/1998 |
| 5656497 | Gene encoding hyperthermostable xylose isomerase from Thermotoga neapolitana The present invention describes the cloning and expression of a hyperthermostable xylose isomerase gene in an industrial host and the heat facilitated purification of the enzyme. The hyperthermostable enzyme has unique features sought by the fructose syru... | 08/12/1997 |
| 5593868 | Method of producing high fructose corn syrup from glucose using noble gases A process for producing high fructose corn syrup from glucose containing syrup, which entails isomerizing the glucose containing syrup through an enzymatic reaction to make high fructose corn syrup, wherein the enzymatic reaction occurs in a gas containin... | 01/14/1997 |
| 5543576 | Production of enzymes in seeds and their use A method of catalyzing in vitro reactions using seeds containing enhanced amounts of enzymes is disclosed. The method involves adding transgenic, non-wild type seeds, preferably in a ground form, to a reaction mixture and allowing the enzymes in the seeds... | 08/06/1996 |
| 5512464 | Method of producing high fructose corn syrup from glucose using noble gases A process for producing high fructose corn syrup from glucose containing syrup, which entails isomerizing the glucose containing syrup through an enzymatic reaction to make high fructose corn syrup, wherein the enzymatic reaction occurs in a gas containin... | 04/30/1996 |
| 5437993 | Preparation of cross-linked glucose isomerase crystals The invention relates to a novel water-insoluble glucose isomerase which is formed by a crystalline enzyme converted to solid form by cross-linking. The invention also concerns a process for the preparation of the novel crystalline glucose isomerase by cr... | 08/01/1995 |
| 5411886 | Xylose isomerase gene of Thermus aquaticus A xylose isomerase gene from Thermus bacteria, such as Thermos aquaticus (ATCC 27634) and a gene having 60% or more of homology to the nucleotide sequence of Thermus aquaticus xylose isomerase gene of FIG. 1-3. A xylose isomerase from Thermus aquaticus ch... | 05/02/1995 |
| 5384257 | Glucose isomerases with an altered pH optimum A method for selecting amino acid residues is disclosed which upon replacement will give rise to an enzyme with an altered pH optimum. The method is specific for metalloenzymes which are inactivated at low pH due to the dissociation of the metal ions. The... | 01/24/1995 |
| 5376536 | Glucose isomerase enzymes and their use New mutant glucose isomerases are provided exhibiting improved properties under application conditions. These glucose isomerases are obtained by expression of a gene encoding said enzyme, having an amino acid sequence which differs at least in one amino a... | 12/27/1994 |
| 5340738 | Modified prokaryotic glucose isomerase enzymes with altered pH activity profiles A method for selecting amino acid residues is disclosed which upon replacement will give rise to an enzyme with an altered pH optimum. The method is specific for metalloenzymes which are inactivated at low pH due to the dissociation of the metal ions. The... | 08/23/1994 |
| 5310665 | Glucose isomerases having altered substrate specificity A method for selecting amino acid residues is disclosed which upon replacement will give rise to an enzyme with an altered substrate specificity. New mutant glucose isomerases with an altered substrate specificity are provided according to this method. Th... | 05/10/1994 |
| 5266475 | Glucose isomerases with improved affinity for D-glucose A genetically engineered glucose isomerase with improved affinity for D-glucose and the method of preparation of such a glucose isomerase are disclosed. The glucose isomerase is obtained by mutagenizing the gene of a naturally occurring glucose isomerase ... | 11/30/1993 |
| 5238826 | Process for manufacturing xylose Process for manufacture of D-xylose characterized by the fact that: in a first step, syrup of D-xylulose is subjected to an enzymatic isomerization in M3 providing a mixture of D-xylose and D-xylulose, in a second step, the abovesaid mixture is subjected... | 08/24/1993 |
| 5219751 | Xylase isomerase purified from Thermotoga maritima and Thermotoga Neapolitana This invention is in the field of glucose isomerization enzymes. More specifically, the invention is directed to a novel xylose isomerase, a process for the preparation of this enzyme, the use of this enzyme in glucose isomerization processes, and glucose... | 06/15/1993 |
| 5177005 | Method for maintaining immobilized glucose isomerase activity during continuous isomerization of glucose to fructose This invention provides a method for the maintenance of continuous activity of an immobilized enzyme reaction by initially underloading an adsorbent with a desired enzyme, monitoring the activity of the enzyme and periodically adding fresh enzyme to maint... | 01/05/1993 |
| 5120650 | Method for producing crystalline glucose isomerase In a process for growing enzyme crystals, small crystals are continuously removed from a crystallizer, dissolved and returned to the crystallizer to maintain a supersaturated state. The method permits the growing of large crystalline enzymes of uniform si... | 06/09/1992 |
| 5041378 | Procaryotic xylose isomerase muteins Xylose isomerase (XI) muteins useful in the conversion of glucose to fructose or xylose to xylulose are obtained in usable amounts by protein structural and recombinant DNA methods, including x-ray crystallography, cloning, computer graphic modeling and s... | 08/20/1991 |
| 5001063 | Treatment of cellulosic ion exchange composites in hot aqueous medium to increase adsorption of macromolecules A composite containing DEAE-cellulose agglomerated with a hydrophobic polymer is treated with tap water, deionized water or a dilute salt solution at a temperature of at least 60° C. for a time sufficient to increase adsorption capacity for charged macro... | 03/19/1991 |
| 4950596 | Stabilization of intracellular enzymes The subject invention concerns a process for stabilizing intact or ruptured microbial cells having glucose isomerase associated therewith. Specifically exemplified is a process for stabilizing glucose isomerase producing cells of a microorganism belonging... | 08/21/1990 |
| 4929556 | Enzyme immobilization with polysulfonium salts Polysulfonium salts that can react with nucleophilic groups and covalently cross-link are used to immobilize enzymes or enzyme-containing cellular material. Some of the polysulfonium salts can both flocculate and covalently cross-link. Replacement of the ... | 05/29/1990 |
| 4920052 | Process for producing glucose isomerase A glucose isomerase useful for the conversion of glucose to fructose can be prepared by growing under aerobic conditions a culture of a species of Microbacterium in a medium containing appropriate nutrients and then recovering the enzyme.... | 04/24/1990 |
| 4894331 | Partial marker cassette mutagenesis of xylose isomerase Constructions and methods for mutagenesis of nucleic acids involve chemical mutagenesis of a cassette comprising a structural gene linked to a non-functional restorable fragment of a marker gene. Mutants are detected by screening for the presence of the r... | 01/16/1990 |
| 4868114 | Method for elevating glutathione levels A method comprising stimulating the biosynthesis of glutathione in mammalian cells by contacting the cells with an effective amount of a compound of the formula: ##STR1## wherein R is a (CHOH)n CH2 OH and wherein n is 1-5, prefe... | 09/19/1989 |