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| Number | Title | Issue Date |
| 4223090 | Reagents for the enzymatic determination of triglycerides A method of determining triglycerides in biological fluids according to an enzymatic reaction. In the reaction, a biological fluid is added to an enzyme reagent comprising a lipase, glycerol dehydrogenase (GDH), pyridine nucleotide (PN), a buffer, a tetra... | 09/16/1980 |
| 4212938 | Reagent and method for the determination of cholesterol Cholesterol is determined by incubating a sample suspected of containing cholesterol in an aqueous medium with cholesterol oxidase and determining either the oxygen consumption, the hydrogen peroxide formed, or cholestenone, as a measure of the initial ch... | 07/15/1980 |
| 4212939 | Substrate solution for carboxylic ester hydrolase determination A clear, stable aqueous reagent solution comprising a mixture of a water insoluble vinyl ester of an alkyl, alkenyl, or alkyl-aryl carboxylic acid, nonionic detergents, inorganic salts, enzyme activators, and a buffer, which when combined with a suitable ... | 07/15/1980 |
| 4202938 | Procedure for the quantitative determination of hydrogen peroxide concentration in aqueous solutions A novel procedure for the quantitative determination of hydrogen peroxide concentration in aqueous solutions is described. This is characterized by reacting hydrogen peroxide with an alcohol in the presence of the catalyst catalase and by reacting the res... | 05/13/1980 |
| 4195126 | Albumin-dye complex for fatty acid determination An albumin-dye complex is prepared containing serum or plasma albumin which is substantially free of endogenous fatty acids and which has been complexed with a fatty acid and dye which dye may be displaced from the albumin-dye complex by free fatty acids.... | 03/25/1980 |
| 4188320 | Ester of higher fatty acid and reagent for determination of activity of lipase An ester of higher fatty acid of the formula: ##STR1## wherein R and M are as defined hereunder, and a reagent for determination of the activity of lipase which contains said ester are disclosed. Such reagent is useful to determine the activity of li... | 02/12/1980 |
| 4188264 | Process for determining bacterial endotoxin and reagents used therefor A process for determining a bacterial endotoxin, which comprises contacting an assay sample with (A) a material selected from the group consisting of an amoebocyte lysate of horseshoe crab an a pro-clotting enzyme separated from the lysate, and (B) a pept... | 02/12/1980 |
| 4184920 | Enzymatic substrate composition adsorbed on a carrier An enzymatic substrate adsorbed on a solid carrier with a large specific surface area of over 50 m2 /g. The enzymatic substrate shall be stable in the absence of the corresponding enzyme and essentially non-soluble in water. The substrate conta... | 01/22/1980 |
| 4179334 | Hydrolysis of protein-bound triglycerides A novel process is described for hydrolyzing protein-bound triglycerides such as blood serum triglycerides comprising contacting sample containing protein-bound triglycerides with a compatible mixture of an enzyme preparation which demonstrates triglyceri... | 12/18/1979 |
| 4168203 | Quantitative analysis of neutral lipids and lecithin A reagent for quantitative analysis of neutral lipids in serum or internal organs comprising a lipase produced by the NRS-400 strain of Rhizopus and an aqueous solvent.... | 09/18/1979 |
| 4141792 | Composition and method for the quantitative determination of phospholipids Phospholipid content in a sample is quantitatively determined by enzymatically converting the phospholipids into choline and then determining the amount of the choline.... | 02/27/1979 |
| 4140579 | Method of testing for phospholipases using a composition containing a uniform dispersion of a phospholipid A uniform dispersion of a phospholipid in an aqueous based, salt containing solution may be obtained by dissolving the phospholipid in an alcohol chosen from the group consisting of methanol, ethanol, propanol, and isopropyl alcohol, and adding the phosph... | 02/20/1979 |
| 4139415 | In vitro method of determining the biological activity of factor Xa inhibitor in blood The present invention concerns an in vitro method for the determination of the plasma inhibitor to activated Factor X (XaI), which is an alpha-2-globulin of molecular weight 58,000-65,000, and which has been termed variously as antithrombin III, heparin c... | 02/13/1979 |
| 4135980 | Choline oxidase A novel enzyme choline oxidase is produced by culturing a microorganism belonging to the genus Arthrobacter, and particularly the species Arthrobacter globiformis B-0577 FERM-P No. 3518, NRRL B-11097, and isolating the choline oxidase thus produced from t... | 01/23/1979 |
| 4123614 | Novel assay reagents The present invention relates to novel assay reagents, their composition, methods of preparation, and use in the detection and measurement of various biological systems and/or components, e.g. enzymes, antibodies, antigens, and periodate concentration. Th... | 10/31/1978 |
| 4066508 | Process and reagent for determining triglycerides Triglycerides are determined by enzymatic saponification with lipase in a buffer in the presence of a carboxylesterase and an alkali metal or alkaline earth metal (10-15 carbon atom) alkyl sulfate, in the additional presence of a compound of the general f... | 01/03/1978 |
| 4056442 | Lipase composition for glycerol ester determination A synergistic composition of microbial lipases from Rhizopus arrhizirs and Candida cylindracea is used for the hydrolysis of glycerol esters such as triglycerides in the determination of glycerol esters present in an aqueous medium such as body fluid.... | 11/01/1977 |
| 4052263 | Production of cholesterol esterase using Nocardia cholesterolicum An improved fermentation method comprises growing Nocardia cholesterolicum in a medium comprising a carbon source, such as glycerol, yeast extract, a nonionic surfactant, an auxiliary carbon source which is also an inducer of cholesterol oxidase, and trac... | 10/04/1977 |
| 4045297 | Triglycerides determination method A method of determining triglycerides in biological fluids which comprises reacting a biological fluid sample with a lipolytic enzyme to liberate free glycerol, reacting said free glycerol with a coupled enzyme series in an oxygenated aqueous solution con... | 08/30/1977 |
| 4043871 | Enzymatic substrate composition adsorbed on a carrier An enzymatic substrate adsorbed on a solid carrier with a large specific surface area of over 50 m2 /g. The enzymatic substrate shall be stable in the absence of the corresponding enzyme and essentially non-soluble in water. The substrate conta... | 08/23/1977 |
| 4041111 | Phosphonate monoesters and method of preparation Synthesis of phosphonate monoesters, as well as the use of such monoesters as substrates for enzymes which normally hydrolyze phosphodiester linkages, and a method for distinguishing between Type I and Type II phosphodiesterase enzymes using such monoeste... | 08/09/1977 |
| 4040908 | Polarographic analysis of cholesterol and other macromolecular substances A micro-method for measurement of sterols such as cholesterol, and other macromolecular substances, is disclosed utilizing enzymes for the conversion of such substances to produce ultimately hydrogen peroxide and measurement of the generated hydrogen pero... | 08/09/1977 |
| 4038146 | Method of determination of serum triglycerides and reagents A serum sample is added to a reaction liquid obtained by adding an aqueous solution of a mixture of a tetrazolium compound and phenazine methosulfate to an enzyme-coenzyme reagent comprising LPL, GDH and NAD thereby effecting hydrolysis reaction of serum ... | 07/26/1977 |
| 4035239 | Enzymic assay for acetate ion An improved enzymic assay and assay composition for acetate ion in a biological fluid (e.g., blood serum or tissue extract) or a solution of fruit juice origin (e.g., wine). The acetate ion is reacted with adenosine triphosphate in the presence of enzymic... | 07/12/1977 |
| 4022667 | Substrate solution for carboxylic ester hydrolase determination A clear, stable aqueous reagent solution comprising a mixture of a water insoluble vinyl ester of an alkyl, alkenyl, or alkyl-aryl carboxylic acid, nonionic detergents, inorganic salts, enzyme activators, and a buffer, which when combined with a suitable ... | 05/10/1977 |
| 4019961 | Analytical enzymatic determination An analytical process for enzymatic determination of a substrate in biological fluids. The fluid sample is reacted first in the absence of the specific kinase but in the presence of an NADH -- regenerating enzymatic system with the reagents needed for the... | 04/26/1977 |
| 4013512 | Method for adsorption and elution of lipid hydrolyzing enzymes A lipid hydrolyzing enzyme-containing substance is brought into contact with an insoluble carrier having a hydrophobic organic compound attached thereto in the form of a hydrophobic residue so that the lipid hydrolyzing enzyme is adsorbed by the hydrophob... | 03/22/1977 |
| 3990946 | Substrate for the determination of desoxy-ribonuclease A desoxy-ribonuclease substrate is prepared by boiling and homogenizing an aqueous dispersion of high molecular weight desoxy-ribonucleic acid (DNA) and 2-amino-7-dimethylamino-3-methyl diphenazthionium chloride (toluidine blue O) until a clear blue-color... | 11/09/1976 |
| 3986930 | Lipase activity determining method and reagent A reagent for determining lipase activity, which comprises as the active ingredient one of the S-acyl compounds of the formula: ##EQU1## WHEREIN N IS AN INTEGER OF 2 TO 4, INCLUSIVE; N' IS AN INTEGER OF 2 TO 4, INCLUSIVE OR OF 9 TO 13, INCLUSIVE; M I... | 10/19/1976 |
| 3983005 | Integral element for the analysis of cholesterol An integral analytical element for analysis of liquids for their cholesterol content is disclosed. The element is of the type which comprises at least two superposed layers including a spreading layer and a reagent layer in fluid contact and, optionally, ... | 09/28/1976 |
| 3932221 | Immunological isozyme determination method This invention relates to a process, to a diagnostic agent employed therein and to the use thereof, for the organ-specific and/or disease-specific diagnosis of the isozyme pattern of an enzyme occurring in multiple molecular configurations in a sample of ... | 01/13/1976 |
