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| Number | Title | Issue Date |
| 5643734 | Methods for protein binding enzyme complementation assays This invention relates to improved methods and novel compositions for enzyme complementation assays for qualitative and quantitative determination of a suspected analyte in a sample. The use of enzyme-acceptor and enzyme-donor polypeptides prepared by rec... | 07/01/1997 |
| 5604091 | Methods for protein binding enzyme complementation This invention relates to improved methods and novel compositions for enzyme complementation assays for qualitative and quantitative determination of a suspected analyte in a sample. The use of enzyme-acceptor and enzyme-donor polypeptides prepared by rec... | 02/18/1997 |
| 5444161 | Substrates for ଲ-galactosidase Galactopyranoside derivative compounds for use as substrates for hydrolysis by enzymes with ଲ-galactosidase activity are provided. The concentration of the reaction products can be measured by spectroscopy. The compounds find particular use in diagn... | 08/22/1995 |
| 5434052 | Complementation assay for drug screening Compounds are evaluated for their binding to naturally occurring receptors, by employing the natural ligand conjugated to an enzyme donor fragment of ଲ-galactosidase for competing with the sample compound for the natural acceptor binding site or in ... | 07/18/1995 |
| 5362625 | Methods and compositions for enzyme complementation assays using the omega region of ଲ-galactosidase The use of omega-acceptor and omega-donor polypeptides (comprising about two-thirds and one-third of the ଲ-galactosidase molecule amino and carboxyl termini, respectively), prepared by recombinant DNA techniques, DNA synthesis, or chemical polypepti... | 11/08/1994 |
| 5244785 | Determination of high molecular weight analytes using a ଲ-galactosidase complementation assay A method for detecting the presence of a high-molecular-weight analyte, which uses a variation on complementation assays, comprising (1) providing an enzyme donor (ED)complex comprising ED coupled to an analyte-specific binding molecule, wherein the ED co... | 09/14/1993 |
| 5244786 | Method of measuring available free thyroxine bending sites The present invention provides an improved assay for measuring thyroxine uptake by thyroxine binding globulin in which thyroxine binding globulin (TBG) activity is measured directly. The method comprises combining a sample in an aqueous solution with an e... | 09/14/1993 |
| 5223393 | Detection of analytes having binding sites for at least two binding moieties The present invention provides reagents for a detectable label for use in specific binding assays. Specifically, modified ଲ-galactosidase enzyme donors (ED) and acceptors (EA) are utilized. Both ED and EA are modified to form separate ED and EA comp... | 06/29/1993 |
| 5212081 | Stabilization of polypeptide fragments against proteolytic degradation Enzyme-donor fragments and other unstructured peptides are stabilized against proteolytic degradation by including a mixture of soluble, random-sequence peptides in the assay medium or in the medium in which the enzyme-donor fragment or other peptide is s... | 05/18/1993 |
| 5212064 | Solid phase non-separation enzyme complementation assay Novel assays employing enzyme fragments which complex to form an active enzyme are provided. The reagents involved are a member of a specific binding pair bound to a solid surface, a first enzyme fragment conjugated to a member of a specific binding pair ... | 05/18/1993 |
| 5188938 | Enzyme quantitation wicking assay Diagnostic assays are provided comprising complementary enzyme fragments of ଲ-galactosidase, where one of the fragments is bound to a support and the other fragment is conjugated to an immunologically cross-reactive epitope of the analyte or complem... | 02/23/1993 |
| 5120653 | Vector comprising DNA sequence coding for enzyme-donor polypeptide This invention relates to improved methods and novel compositions for enzyme complementation assays for qualitative and quantitative determination of a suspected analyte in a sample. The use of enzyme-acceptor and enzyme-donor polypeptides prepared by rec... | 06/09/1992 |
| 5037735 | Visual discrimination qualitative enzyme complementation assay A ଲ-galactosidase complementation assay for determining the presence of an analyte which is a member of a specific binding pair (sbp) in a sample is provided, which assay permits visual discrimination between those samples wherein the analyte is pre... | 08/06/1991 |
| 5032503 | Liquid single reagent for air enzyme complementation assay A method is provided for combining normally interacting reagents in a liquid single reagent and preventing complex formation using a surfactant and then reversing the inhibition by adding a cyclodextrin. The method finds particular use in diagnostic immun... | 07/16/1991 |
| 4956274 | Reagent stabilization in enzyme-donor and acceptor assay A method of stabilizing ଲ-galactosidase peptide fragments against storage degradation for use in a complementation assay which comprises storing the peptide fragment in a storage medium containing an ionic surfactant or a surfactant derived from a s... | 09/11/1990 |
| 4950612 | Peroxy acid pretreatment in vitamin B12 assay Serum samples for vitamin B12 analysis can be pretreated using a combination of peroxy acid and dithiothreitol. The pretreatment makes vitamin B12 bound to serum binding proteins available for analysis by any of a variety of currentl... | 08/21/1990 |
| 4708929 | Methods for protein binding enzyme complementation assays This invention relates to improved methods and novel compositions for enzyme complementation assays for qualitative and quantitative determination of a suspected analyte in a sample. The use of enzyme-acceptor and enzyme-donor polypeptides prepared by rec... | 11/24/1987 |
