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Assignee: Genencor, Inc.


Location: SouthSanFrancisco, CA
No. of patents: 16

NumberTitleIssue Date
5441882Method for preparing modified subtilisins
There are described methods for making mutant subtilisins, the methods comprising obtaining a DNA fragment from a Bacillus subtilisin and introducing a mutation into the fragment by substituting at least one amino acid, transforming a suitable host cell w...
08/15/1995
5411873Process for producing heterologous polypeptides
Processes for producing various heterologous polypeptides which when expressed are either incorrectly processed and hence asssociated with the surface of the host cell or are not processed to mature form. More specifically, processes for the production of...
05/02/1995
5378621Killing cells without lysis in a method for enzyme recovery from a fermentation broth
This invention provides a method for killing fungal cells without lysing in fermentation processes in order to prepare the fermentation mixture for processing to recover or extract an extracellularly expressed enzyme from the fermentation mixture. A prefe...
01/03/1995
5316941Non-human carbonyl hydrolase mutants, DNA sequences and vectors encoding same and hosts transformed with said vectors
There are described certain DNA sequences which encode subtilisins wherein the amino acid sequence of such substilisins has been modified at a position equivalent to +225 in Bacillus amyloliquefaciens, such that an amino acid selected from the group consi...
05/31/1994
5264366Protease deficient bacillus
There are described, normally sporulating mutant Bacillus strain(s) which produce no detectable proteolytic activity during any phase of its growth. The absence of detectable proteolytic activity is due to the deletion of one or more codons specifying the...
11/23/1993
5155033Subtilisins modified at position 225 resulting in a shift in catalytic activity
There are described certain subtilisins wherein the amino acid sequence of such subtilisins has been modified at a position equivalent to +225 in Bacillus amyloliquefaciens, such that an amino acid selected from the group consisting of alanine, leucine, m...
10/13/1992
4981611Cutinase cleaning compositions
This invention relates to cleaning compositions and methods for using them. Particularly, the invention relates to compositions comprising a surfactant and a cutinase enzyme. A preferred cutinase is derived from Pseudomonas putida ATCC 53552. Excellent cl...
01/01/1991
4927644Preferential entrainment of enzymes in cheese curds
The invention relates to a novel method for preferentially entraining a lipase or protease substantially into cheese curds when making curds and whey. An insoluble enzyme particle size of at least about 0.20 microns is selected or formed, and upon curd fo...
05/22/1990
4795101Use of cellulase in a method of wet milling a starch-containing grain
The invention relates to a method of milling grain, especially corn, comprising cleaning the grain, steeping the grain in water to soften it, and then milling the grain with a cellulase enzyme....
01/03/1989
4760025Modified enzymes and methods for making same
A cloned subtilisin gene has been modified at specific sites to cause amino acid substitutions at certain spots in the enzyme. The modified enzyme, preferably produced by Bacillus, is useful in combination with detergents....
07/26/1988
4749511Contact lens cleaning solutions containing endoproteinase lys-C
Contact lens cleaning compositions and a method which comprises treating soft contact lenses with general purpose proteases in combination with endoproteinase lys-C are effective in dissolving away and hydrolyzing lysozyme, the major protein component of ...
06/07/1988
4726954Lipolytic enzyme derived from a aspergillus microorganism having an accelerating effect on cheese flavor development
The present invention relates to a novel lipolytic enzyme derived from a novel Aspergillus microorganism. Cheese aged in the presence of a low concentration of this lipolytic enzymes ripens faster than with conventional lipolytic enzymes and without any a...
02/23/1988
4721676Novel thermophile isolate having thermostable hydrolytic activity
This application represents an invention based upon the discovery of a novel thermophilic organism, its isolation from natural source and to the unique thermostable hydrolytic activity produced by this bacterium, this activity being useful for industrial ...
01/26/1988
4716911Method for protein removal from tobacco
An improved process for removing insoluble nitrogen-containing compounds from cured tobacco uses alkali or a combination of protease and nonprotease depolymerase, rather than simple protease extraction. The method of the invention is more efficient and results...
01/05/1988
4636468Lipolytic enzyme derived from a aspergillus microorganism having an accelerating effect on cheese flavor development
The present invention relates to a novel lipolytic enzyme derived from a novel Aspergillus microorganism. Cheese aged in the presence of a low concentration of this lipolytic enzymes ripens faster than with conventional lipolytic enzymes and without any a...
01/13/1987
4618579Raw starch saccharification
A multi-step process is provided for solubilizing and saccharifying granular starch slurries of between 20 and 60% d.s. starch. In a first step, an enzyme mixture exhibiting raw starch hydrolyzing activity is added to the slurry and maintained in contact ...
10/21/1986
 
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