Assignee: Diversa Corporation

Location: San Diego, CA
No. of patents: 39

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Number	Title	Issue Date
7232677	Phytase expression systems and methods of making and using them The invention provides a purified or recombinant phytase enzyme (SEQ ID NO:2) initially derived from Escherichia coli B. The enzyme has a molecular weight of about 47.1 kilodaltons and has phytase activity (SEQ ID NO:2). The enzyme can be produced from native...	06/19/2007
7232672	P450 enzymes, nucleic acids encoding them and methods of making and using them The invention is directed to polypeptides having P450 activity, polynucleotides encoding the polypeptides, antibodies that bind to these polypeptides, and methods for making and using these polynucleotides and polypeptides. The P450 enzymes can be used to catalyze t...	06/19/2007
7226771	Phospholipases, nucleic acids encoding them and methods for making and using them The invention provides novel polypeptides having phospholipase activity, including, e.g., phospholipase A, B, C and D activity, patatin activity, lipid acyl hydrolase (LAH) activity, nucleic acids encoding them and antibodies that bind to them. Industrial methods, e...	06/05/2007
7138260	Microbially-expressed thermotolerant phytase for animal feed The invention provides methods for making and using thermotolerant phytases, e.g., a method of using a thermotolerant phytase in feed and food processing and feed or food products comprising a thermotolerant phytase. ...	11/21/2006
7135323	Microbially expressed thermotolerant phytase for animal feed The invention provides methods for making and using thermotolerant phytases, e.g., a method of using a thermotolerant phytase in feed and food processing and feed or food products comprising a thermotolerant phytase. ...	11/14/2006
7078035	Phytases, nucleic acids encoding them and methods for making and using them The invention provides isolated and recombinant phytase enzymes. In one aspect, the phytases are produced by modification of the wild type appA of E. coli. The enzyme can be produced from recombinant host cells. The phytases of the invention can be used to ai...	07/18/2006
7078504	Enzymes having dehalogenase activity and methods of use thereof The invention relates to haloalkane dehalogenases and to polynucleotides encoding alkane dehalogenases. In addition methods of designing new dehalogenases and of use thereof are also provided. The dehalogenases have increased activity and at increased pH and tempera...	07/18/2006
7056703	Polypeptides having polymerase activity and methods of use thereof The invention relates to thermostable polymerases that have polymerase activity temperatures in the range from 90° C. up to 113° C., such as those derived from Pyrolobus fumaria, and to polynucleotides encoding the polymerases In addition, methods of design...	06/06/2006
7056691	Assays for identifying α-galactosidases The invention relates to α-galactosidase and to polynucleotides encoding the α-galactosidase. In addition methods of designing new α-galactosidases and method of use thereof are also provided. The α-galactosidases have increased activity and stability at increas...	06/06/2006
7049101	Enzymes having high temperature polymerase activity and methods of use thereof The invention relates to thermostable polymerases that have polymerase activity temperatures in the range from 90° C. up to 113° C., such as those derived from Pyrolobus fumaria, and to polynucleotides encoding the polymerases In addition, methods of design...	05/23/2006
7033781	Whole cell engineering by mutagenizing a substantial portion of a starting genome, combining mutations, and optionally repeating An invention comprising cellular transformation, directed evolution, and screening methods for creating novel transgenic organisms having desirable properties. Thus in one aspect, this invention relates to a method of generating a transgenic organism, such as a micr...	04/25/2006
7019827	GigaMatrix holding tray having through-hole wells A GigaMatrix plate for holding a large number of small-volume fluid samples includes a base for supporting a plurality of substantially parallel, elongated capillary tubes. Each tube defines a lumen that extends through the base, and each lumen has an aspect ratio g...	03/28/2006
7018793	Combinatorial screening of mixed populations of organisms Provided is a method of screening gene libraries derived from a mixed population of organisms for a bioactivity or biomolecule of interest. The mixed population of organisms can be a cultured population or an uncultured population from, for example, the environment....	03/28/2006
6987016	α-galactosidases and methods for making and using them The invention relates to α-galactosidase and to polynucleotides encoding the α-galactosidase. In addition methods of designing new α-galactosidases and method of use thereof are also provided. The α-galactosidases have increased activity and stability at increas...	01/17/2006
6987009	αgalactosidases and methods of using them A thermostable alpha-glycosidase derived from various Thermococcus, alcaliphilus AEDII12RA is disclosed. The enzymes are produced from native or recombinant host cells and can be utilized in the food processing industry. ...	01/17/2006
6979733	Epoxide hydrolases, nucleic acids encoding them and methods of making and using them The invention is directed to polypeptides having epoxide hydrolase activity, polynucleotides encoding the polypeptides, antibodies that bind to these polypeptides, and methods for making and using these polynucleotides and polypeptides. The epoxide hydrolases are us...	12/27/2005
6972183	Capillary array-based enzyme screening A process for screening an expression library to identify clones expressing enzymes having a desired activity is provided. The process involves first generating from genomic DNA samples of one or more microorganisms an expression library comprising a plurality of re...	12/06/2005
6949369	α-galactosidases and methods for making and using them The invention relates to α-galactosidase and to polynucleotides encoding the α-galactosidase. In addition methods of designing new α-galactosidases and method of use thereof are also provided. The α-galactosidases have increased activity and stability at increas...	09/27/2005
6943001	Epoxide hydrolases, nucleic acids encoding them and methods for making and using them The invention is directed to polypeptides having epoxide hydrolase activity, polynucleotides encoding the polypeptides, antibodies that bind to these polypeptides, and methods for making and using these polynucleotides and polypeptides. The epoxide hydrolases are us...	09/13/2005
6939689	Exonuclease-mediated nucleic acid reassembly in directed evolution This invention provides methods of obtaining novel polynucleotides and encoded polypeptides by the use of non-stochastic methods of directed evolution (DirectEvolution™). A particular advantage of exonuclease-mediated reassembly methods is the ability to reassembl...	09/06/2005
6918738	Stackable sample holding plate with robot removable lid A holding plate includes a flat base member with a surface that is formed with a plurality of stations (wells or depressions) for holding specimens. A panel extends downwardly from the edge of the surface to create a concavity for receiving a lid member therein. The...	07/19/2005
6872526	High throughput screening for novel Bioactivities Disclosed is a process for identifying clones having a specified activity of interest, which process comprises (i) generating one or more expression libraries derived from nucleic acid directly isolated from the environment; and (ii) screening said libraries utilizi...	03/29/2005
6866824	Capillary array-based sample screening Provided are methods of screening and identification of bioactivities and bioactive molecules of interest using a capillary array system. More specifically, disclosed are methods of using optical detection and capillary array-based techniques for screening libraries...	03/15/2005
6855365	Recombinant bacterial phytases and uses thereof A purified and modified phytase enzyme from Escherichia coli K12 appA phytase is provided. The enzyme has phytase activity and improved thermal tolerance as compared with the wild-type enzyme. In addition, the enzyme has improved protease stability at low pH....	02/15/2005
6849395	Gene cluster screening of clones having DNA from mixed populations of organisms Disclosed is a process of screening clones having DNA from an uncultivated microorganism for a specified protein, e.g. enzyme, activity by screening for a specified protein, e.g. enzyme, activity in a library of clones prepared by (i) recovering DNA from a DNA popul...	02/01/2005
6846627	Screening for novel compounds which regulate biological interactions Disclosed is a process for identifying compounds having a specified activity of interest, which process comprises (i) introducing interacting molecules into a host cell under conditions to generate or repress a detectable signal; and (ii) introducing a third compoun...	01/25/2005
6806048	Method for high throughput screening of an environmental library Disclosed is a process for identifying clones having a specified activity of interest, which process comprises (i) generating one or more expression libraries derived from nucleic acid directly isolated from the environment; and (ii) screening said libraries utilizi...	10/19/2004
6798520	Method for intensifying the optical detection of samples that are held in solution in the through-hole wells of a holding tray A system and method for optically detecting samples held in a solution requires the use of a holding plate that has as many as one-thousand through-hole wells, or more. The solution is suspended in these through-hole wells under surface tension between opposed surfa...	09/28/2004
6794127	Capillary array-based sample screening Provided are methods of screening and identification of bio activities and bioactive molecules of interest using a capillary array system. More specifically, disclosed are methods of using optical detection and capillary array-based techniques for screening librarie...	09/21/2004
6790605	Methods for obtaining a desired bioactivity or biomolecule using DNA libraries from an environmental source Disclosed is a process for obtaining an enzyme having a specified enzyme activity derived from a heterogeneous DNA population by screening, for the specified enzyme activity, a library of clones containing DNA from the heterogeneous DNA population which have been ex...	09/14/2004
6773900	End selection in directed evolution This invention provides methods of obtaining novel polynucleotides and encoded polypeptides by the use of non-stochastic methods of directed evolution (DirectEvolution™). A particular advantage of end-selection-based methods is the ability to recover full-length p...	08/10/2004
6764835	Saturation mutageneis in directed evolution Disclosed is a rapid and facilitated method of producing from a parentlal template polynucleotide, a set of mutagenized progeny polynculeotides whereby at each original codon position there is produced at least one substitute codon encoding each of the 20 naturally ...	07/20/2004
6764818	Device for effecting heat transfer with a solution held in a through-hole well of a holding tray A holding plate for selectively heating and cooling samples in a solution has two opposing surfaces, and a plurality of cylindrically-shaped through-hole wells for holding the samples. Each well extends between the two surfaces of the holding plate, and has an aspec...	07/20/2004
6740506	End selection in directed evolution This invention provides methods of obtaining novel polynucleotides and encoded polypeptides by the use of non-stochastic methods of directed evolution (DirectEvolution™). A particular advantage of end-selection-based methods is the ability to recover full-length p...	05/25/2004
6720014	Phytase-containing foodstuffs and methods of making and using them A purified recombinant phytase enzyme derived from Escherichia coli B. The enzyme has a molecular light of about 47.1 kilodaltons and has phytase activity. The enzyme can be produced from native or recombinant host cells and can be used to aid in the digestio...	04/13/2004
6713281	Directed evolution of thermophilic enzymes Thermostable enzymes are subjected to mutagenesis to produce a thermophilic enzyme which is stable at thermophilic temperature and which has increased activities at least two-fold higher than the activity of the wild-type thermostable enzyme at lower temperatures, w...	03/30/2004
6713282	End selection in directed evolution This invention provides methods of obtaining novel polynucleotides and encoded polypeptides by the use of non-stochastic methods of directed evolution (DirectEvolution™). A particular advantage of end-selection-based methods is the ability to recover full-length p...	03/30/2004
6713279	Non-stochastic generation of genetic vaccines and enzymes This invention provides methods of obtaining novel polynucleotides and encoded polypeptides by use of non-stochastic methods of directed evolution (DirectEvolution™). These methods include non-stochastic polynucleotide site-saturation mutagenesis (Gene Site Satura...	03/30/2004
6709841	Exonuclease-mediated gene assembly in directed evolution A directed evolution process comprising novel methods for generating improved progeny molecules having desirable properties, including, for example, a method for rapid and facilitated production from a parental polynucleotide template, of a set of mutagenized progen...	03/23/2004

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Assignee: Diversa Corporation