US Classes435/173.7, Lytic effect produced (e.g., disruption of cell membrane for release of subcellular parts; e.g., nucleic acids, etc.)307/106, WAVE FORM OR WAVE SHAPE DETERMINATIVE OR PULSE-PRODUCING SYSTEMS435/294.1Vessels or trays in series
International ClassesC12M 1/42
A device, system and method for treating biological cells includes a voltage source, a half-controlled bridge connected to the voltage source, and a load connected across the half-controlled bridge. The half-controlled bridge includes a first switch, a second switch, a first diode and a second diode. The load includes an inductor connected in parallel with a cell or chamber. A controller is connected to the first and second switches and operates the first switch and the second switch to selectively generate one or more bipolar pulses, wherein each bipolar pulse comprises a positive polarity voltage pulse and a negative polarity voltage pulse with a negligible delay between the positive polarity voltage pulse and the negative polarity voltage pulse.