Abstract text

The present invention describes a detection device based on the surface plasmon resonance effect, comprising: (1) a rotational microfluidic substrate (40) with channels (45), valves (50) and reservoirs (41,44) and at least one Detection Zone (42) wherein said Detection Zone comprises a Detection Surface (DS) built on top of a dif tractive thin electrically conductive layer, —(2) a system comprising a light emitter (20) and a light detector (30) capable of transducing the occurrence of events near the DS by exploiting the surface plasmon resonance effect in the diffractive conductive layer, —(3) a mechanism for controlling the rotation speed, duration and positioning of the rotational microfluidic substrate, in order to move a predefined liquid volume from an initial reservoir into a Detection Zone and finally into a final reservoir. The sensor described in the present invention enables the determination of the concentration of specific chemical and/or biological substances present at the DS or present in the fluid near the DS.